Banham Alison H, Lyne Linden, Scase Timothy J, Blacklaws Barbara A
LRF Haemato-oncology Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, UK.
Vet Immunol Immunopathol. 2009 Feb 15;127(3-4):376-81. doi: 10.1016/j.vetimm.2008.10.328. Epub 2008 Nov 5.
A population of primarily CD4(+)CD25(+) regulatory T cells (Tregs), that have a critical role in maintaining the balance between tolerance and immunity, have been identified through their ability to provide protection against autoimmune disease. There is considerable interest in further exploring the role that Tregs play in autoimmune disease, cancer, and in regulating the immune response to pathogens. Currently the best single marker for labelling Tregs is the forkhead transcription factor FOXP3. Consistent with its essential functional role, sequence alignment showed that the FOXP3 protein is highly conserved across mammalian species. Lymphoid tissues were analysed for nuclear Foxp3 protein expression by immunohistochemistry to evaluate the utility of monoclonal antibodies raised to the human FOXP3 protein for labelling Foxp3(+) Tregs in other mammalian species. The T-cell specificity of those anti-FOXP3 antibodies that gave the most effective staining on each species was confirmed by double labelling with FOXP3 and CD3. Antibodies 236A/E7 and 206D/B1 showed least reactivity with other species, while 259D/C7 commonly exhibited non-specific nuclear staining of non-human lymphoid tissues. Antibodies 86D/D6, 150D/E4 and 157B/F4 are recommended as those which are most effective for labelling Foxp3(+) Tregs in studies utilising animal models.
一群主要为CD4(+)CD25(+)调节性T细胞（Tregs），在维持耐受与免疫之间的平衡中起关键作用，已通过其预防自身免疫性疾病的能力得以识别。人们对进一步探索Tregs在自身免疫性疾病、癌症以及调节对病原体的免疫反应中所起的作用有着浓厚兴趣。目前，用于标记Tregs的最佳单一标志物是叉头转录因子FOXP3。与其重要的功能作用相一致，序列比对显示FOXP3蛋白在哺乳动物物种间高度保守。通过免疫组织化学分析淋巴组织中核Foxp3蛋白的表达，以评估针对人FOXP3蛋白产生的单克隆抗体用于标记其他哺乳动物物种中Foxp3(+) Tregs的效用。通过用FOXP3和CD3进行双重标记，证实了那些在每个物种上染色效果最有效的抗FOXP3抗体的T细胞特异性。抗体236A/E7和206D/B1与其他物种的反应性最低，而259D/C7通常对非人类淋巴组织表现出非特异性核染色。在利用动物模型的研究中，推荐使用抗体86D/D6、150D/E4和157B/F4，因为它们在标记Foxp3(+) Tregs方面最为有效。
