Roncador Giovanna, Engel Pablo, Maestre Lorena, Anderson Amanda P, Cordell Jacqueline L, Cragg Mark S, Šerbec Vladka Č, Jones Margaret, Lisnic Vanda J, Kremer Leonor, Li Demin, Koch-Nolte Friedrich, Pascual Núria, Rodríguez-Barbosa Jose-Ignacio, Torensma Ruurd, Turley Helen, Pulford Karen, Banham Alison H
a Monoclonal Antibody Unit; Spanish National Cancer Research Center ; Madrid , Spain.
b Immunology Unit, Department of Cell Biology; Immunology and Neurosciences ; Medical School, University of Barcelona ; Spain.
MAbs. 2016;8(1):27-36. doi: 10.1080/19420862.2015.1100787. Epub 2015 Sep 29.
Antibodies are widely exploited as research/diagnostic tools and therapeutics. Despite providing exciting research opportunities, the multitude of available antibodies also offers a bewildering array of choice. Importantly, not all companies comply with the highest standards, and thus many reagents fail basic validation tests. The responsibility for antibodies being fit for purpose rests, surprisingly, with their user. This paper condenses the extensive experience of the European Monoclonal Antibody Network to help researchers identify antibodies specific for their target antigen. A stepwise strategy is provided for prioritising antibodies and making informed decisions regarding further essential validation requirements. Web-based antibody validation guides provide practical approaches for testing antibody activity and specificity. We aim to enable researchers with little or no prior experience of antibody characterization to understand how to determine the suitability of their antibody for its intended purpose, enabling both time and cost effective generation of high quality antibody-based data fit for publication.
抗体作为研究/诊断工具和治疗药物被广泛应用。尽管提供了令人兴奋的研究机会，但众多可用抗体也让人面临眼花缭乱的选择。重要的是，并非所有公司都符合最高标准，因此许多试剂无法通过基本的验证测试。令人惊讶的是，确保抗体适用于特定目的的责任在于其使用者。本文总结了欧洲单克隆抗体网络的丰富经验，以帮助研究人员识别针对其靶抗原的特异性抗体。提供了一个逐步的策略，用于对抗体进行优先级排序，并就进一步的必要验证要求做出明智的决策。基于网络的抗体验证指南提供了测试抗体活性和特异性的实用方法。我们旨在使几乎没有或完全没有抗体表征经验的研究人员能够理解如何确定其抗体是否适合其预期用途，从而能够高效地生成高质量的、适合发表的基于抗体的数据。
