Fonseca-Salamanca F, Nogal-Ruiz J J, García-Sánchez R N, Bolas-Fernandez F, Jiménez S, Alamo R, Gárate T, Martínez-Fernandez A R
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n, Madrid, Spain.
Vet Parasitol. 2009 Feb 23;159(3-4):222-4. doi: 10.1016/j.vetpar.2008.10.040. Epub 2008 Oct 22.
Trichinella spiralis and Trichinella britovi live in apparent sympatry among wild fauna of the Iberian Peninsula. In the present study 105 Trichinella isolates from wild mammals were typed by inter-sequence simple repeat PCR (ISSR-PCR). All isolates identified as T. spiralis were indistinguishable from the ISS48 reference strain. Among those belonging to T. britovi, four variations were clearly distinguishable; two of them, ISS11 C-76 and ISS86 MON, had been previously detected while the ISS2 reference strain and Trichinella Rioja 3, (MVUL/SP/02/R3) had not been reported before. The newly distinguished genotype of T. britovi was analyzed by ISSR-PCR, multiplex-PCR, UARR sequencing, and single larva cross-breeding with the other T. britovi genotypes including Trichinella T8 (ISS49). Among all of them, the ISS11 and ISS2 isolates were found to be the most frequent. The uniformity found within T. spiralis isolates is consistent with its recent introduction in Iberian Peninsula, whereas the presence of four variations within T. britovi suggests that this species is an endemic species. Orographical diversity of the West-End of Eurasian Region could act to preserve population diversity observed within T. britovi.
旋毛虫和布氏旋毛虫在伊比利亚半岛野生动物中明显共存。在本研究中，通过序列间简单重复PCR（ISSR-PCR）对105株来自野生哺乳动物的旋毛虫分离株进行了分型。所有鉴定为旋毛虫的分离株与ISS48参考菌株无法区分。在属于布氏旋毛虫的分离株中，有四种变异明显可区分；其中两种，即ISS11 C-76和ISS86 MON，此前已被检测到，而ISS2参考菌株和里奥哈旋毛虫3（MVUL/SP/02/R3）此前尚未见报道。通过ISSR-PCR、多重PCR、UARR测序以及与包括旋毛虫T8（ISS49）在内的其他布氏旋毛虫基因型进行单幼虫杂交，对新区分的布氏旋毛虫基因型进行了分析。在所有这些基因型中，ISS11和ISS2分离株最为常见。旋毛虫分离株中发现的一致性与其最近引入伊比利亚半岛相符，而布氏旋毛虫中存在四种变异表明该物种是一种地方特有物种。欧亚地区西端的地形多样性可能有助于保护在布氏旋毛虫中观察到的种群多样性。
