Apel Anja, Groth Ariane, Schlesinger Sabine, Bruns Helge, Schemmer Peter, Büchler Markus W, Herr Ingrid
Molecular OncoSurgery Group, University of Heidelberg and German Cancer Research Center, Heidelberg, Germany.
Exp Cell Res. 2009 Feb 1;315(3):498-507. doi: 10.1016/j.yexcr.2008.11.013. Epub 2008 Dec 3.
Great hope is set in the use of mesenchymal stem cells for gene therapy and regenerative medicine. Since the frequency of this subpopulation of stem cells in bone marrow is low, mesenchymal stem cells are expanded ex vivo and manipulated prior to experimental or clinical use. Different methods for isolation and expansion are available, but the particular effect on the stem cell character is unclear. While the isolation of mesenchymal stem cells by density centrifugation followed by selection of the plastic adherent fraction is frequently used, the composition of expansion media differs. Thus, in the present study we cultured mesenchymal stem cells isolated from five healthy young volunteers in three widely used expansion media and performed a detailed analysis of the effect on morphology, proliferation, clonogenicity, passaging, differentiation and senescence. By this way we clearly show that the type of expansion medium used determines the stem cell character and time of senescence which is critical for future gene therapeutic and regenerative approaches using mesenchymal stem cells.
间充质干细胞在基因治疗和再生医学中的应用寄予了人们很大的希望。由于骨髓中这种干细胞亚群的频率较低，间充质干细胞在进行实验或临床应用之前需在体外进行扩增和处理。目前有不同的分离和扩增方法，但这些方法对干细胞特性的具体影响尚不清楚。虽然通过密度离心分离间充质干细胞，随后选择贴壁部分的方法经常被使用，但扩增培养基的成分各不相同。因此，在本研究中，我们使用三种广泛使用的扩增培养基培养从五名健康年轻志愿者中分离出的间充质干细胞，并对其对形态、增殖、克隆形成能力、传代、分化和衰老的影响进行了详细分析。通过这种方式，我们清楚地表明，所使用的扩增培养基类型决定了干细胞特性和衰老时间，这对于未来使用间充质干细胞的基因治疗和再生方法至关重要。
