Hill April A, Lipert Robert J, Fritz James S, Porter Marc D
Department of Chemistry, Institute for Combinatorial Discovery, Ames Laboratory-USDOE, Iowa State University, Ames, IA 50011, USA.
Talanta. 2009 Feb 15;77(4):1405-8. doi: 10.1016/j.talanta.2008.09.025. Epub 2008 Sep 24.
Formaldehyde has been detected in drinking water supplies across the globe and on board NASA spacecraft. A rapid, simple, microgravity-compatible technique for measuring this contaminant in water supplies using colorimetric-solid phase extraction (C-SPE) is described. This method involves collecting a water sample into a syringe by passage through a cartridge that contains sodium hydroxide, to adjust pH, and Purpald, which is a well-established colorimetric reagent for aldehydes. After completing the reaction in the syringe by agitating for 2 min on a shaker at 400 rpm, the 1.0-mL alkaline sample is passed through an extraction disk that retains the purple product. The amount of concentrated product is then measured on-disk using diffuse reflectance spectroscopy, and compared to a calibration plot generated from Kubelka-Munk transformations of the reflectance data at 700 nm to determine the formaldehyde concentration. This method is capable of determining formaldehyde concentrations from 0.08 to 20 ppm with a total work-up time of less than 3 min using only 1-mL samples.
全球各地的饮用水供应以及美国国家航空航天局（NASA）的航天器上均检测到了甲醛。本文描述了一种快速、简单且适用于微重力环境的技术，该技术利用比色 - 固相萃取（C - SPE）法来测量供水中的这种污染物。此方法包括通过一个装有氢氧化钠（用于调节pH值）和普尔醛（一种成熟的醛类比色试剂）的滤筒，将水样收集到注射器中。在注射器中于振荡器上以400转/分钟的速度搅拌2分钟完成反应后，将1.0毫升碱性样品通过一个能保留紫色产物的萃取盘。然后使用漫反射光谱法在盘上测量浓缩产物的量，并与通过对700纳米处反射率数据进行库贝尔卡 - 芒克变换生成的校准曲线进行比较，以确定甲醛浓度。该方法仅使用1毫升样品，能够在不到3分钟的总处理时间内测定0.08至20 ppm的甲醛浓度。
