Nigam Preeti, Mohan Swati, Kundu Subir, Prakash Rajiv
School of Biochemical Engineering, Institute of Technology, Banaras Hindu University, Varanasi, India.
Talanta. 2009 Feb 15;77(4):1426-31. doi: 10.1016/j.talanta.2008.09.026. Epub 2008 Sep 25.
Cefotaxime a third generation cephalosporin drug estimation in nanomolar concentration range is demonstrated for the first time in aqueous and human blood samples using novel Schiff base octahedral Zn(II) complex. The cefotaxime electrochemistry is studied over graphite paste and Zn(II) complex modified graphite paste capillary electrodes in H(2)SO(4) (pH 2.3) using cyclic voltammetry and differential pulse voltammetry. Cefotaxime enrichment is observed over Zn(II) complex modified graphite paste electrode probably due to interaction of functional groups of cefotaxime with Zn(II) complex. Possible interactions between metal complex and cefotaxime drug is examined by UV-vis and electrochemical quartz crystal microbalance (EQCM) techniques and further supported by voltammetric analysis. Differential pulse voltammetry (DPV) with modified electrode is applied for the determination of cefotaxime in acidified aqueous and blood samples. Cefotaxime estimation is successfully demonstrated in the range of 1-500 nM for aqueous samples and 0.1-100 microM in human blood samples. Reproducibility, accuracy and repeatability of the method are checked by triplicate reading for large number of samples. The variation in the measurements is obtained less than 10% without any interference of electrolyte or blood constituents.
首次使用新型席夫碱八面体锌（II）配合物在水性和人血样品中证明了纳摩尔浓度范围内头孢噻肟（一种第三代头孢菌素药物）的测定。在硫酸（pH 2.3）中，使用循环伏安法和差分脉冲伏安法在石墨糊和锌（II）配合物修饰的石墨糊毛细管电极上研究了头孢噻肟的电化学。在锌（II）配合物修饰的石墨糊电极上观察到头孢噻肟的富集，这可能是由于头孢噻肟的官能团与锌（II）配合物的相互作用。通过紫外可见光谱和电化学石英晶体微天平（EQCM）技术研究了金属配合物与头孢噻肟药物之间可能的相互作用，并通过伏安分析进一步得到支持。使用修饰电极的差分脉冲伏安法（DPV）用于测定酸化水性和血液样品中的头孢噻肟。在水性样品中，头孢噻肟的测定范围为1-500 nM，在人血样品中为0.1-100 μM，已成功得到证明。通过对大量样品进行三次重复读数来检查该方法的重现性、准确性和重复性。在没有电解质或血液成分干扰的情况下，测量的变化小于10%。
