Rawe Vanesa Y, Chemes Héctor
Centro de Estudios en Ginecología y Reproducción (CEGyR), Buenos Aires, Argentina.
Methods Mol Biol. 2009;518:189-206. doi: 10.1007/978-1-59745-202-1_14.
Understanding the cellular events during fertilization in mammals is a major challenge that can contribute to the improvement of future infertility treatments in humans and reproductive performance in farm animals. Of special interest is the role of the oocyte and sperm cytoskeleton during the initial interaction between gametes. The aim of this chapter is to describe methods for studying cytoskeletal features during in vitro fertilization after intracytoplasmic sperm injection (ICSI) in humans. The following protocols will provide a detailed description of how to perform immunodetection and imaging of human eggs, zygotes, and sperm by fluorescence (confocal and epifluorescence) and electron microscopy.
了解哺乳动物受精过程中的细胞事件是一项重大挑战，这有助于改善未来人类的不孕治疗以及提高农场动物的繁殖性能。特别值得关注的是卵母细胞和精子细胞骨架在配子初始相互作用过程中的作用。本章的目的是描述在人类卵胞浆内单精子注射（ICSI）后体外受精过程中研究细胞骨架特征的方法。以下方案将详细描述如何通过荧光（共聚焦和落射荧光）和电子显微镜对人类卵子、受精卵和精子进行免疫检测和成像。
