Ruggeri Elena, DeLuca Keith F, Galli Cesare, Lazzari Giovanna, DeLuca Jennifer G, Carnevale Elaine M
Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, 1601 Campus Delivery, Fort Collins, CO 80523, USA.
Department of Biochemistry and Molecular Biology, College of Natural Sciences, Colorado State University, 1870 Campus Delivery, Fort Collins, CO 80523, USA.
Reprod Fertil Dev. 2015 Jul;27(6):944-56. doi: 10.1071/RD14468.
Intracytoplasmic sperm injection (ICSI) is an established method to fertilise equine oocytes, but not all oocytes cleave after ICSI. The aims of the present study were to examine cytoskeleton patterns in oocytes after aging in vitro for 0, 24 or 48h (Experiment 1) and in potential zygotes that failed to cleave after ICSI of oocytes from donors of different ages (Experiment 2). Cytoplasmic multiasters were observed after oocyte aging for 48h (P<0.01). A similar increase in multiasters was observed with an increased interval after ICSI for young mares (9-13 years) but not old (20-25 years) mares. Actin vesicles were observed more frequently in sperm-injected oocytes from old than young mares. In the present study, multiasters appeared to be associated with cell aging, whereas actin vesicles were associated with aging of the oocyte donor.
胞浆内单精子注射（ICSI）是一种成熟的使马属动物卵母细胞受精的方法，但并非所有卵母细胞在ICSI后都会发生卵裂。本研究的目的是检测体外老化0、24或48小时后卵母细胞中的细胞骨架模式（实验1），以及不同年龄供体的卵母细胞在ICSI后未能卵裂的潜在合子中的细胞骨架模式（实验2）。卵母细胞老化48小时后观察到细胞质多星体（P<0.01）。年轻母马（9 - 13岁）在ICSI后随着时间间隔增加，多星体有类似增加，但老年母马（20 - 25岁）没有。老年母马经精子注射的卵母细胞中肌动蛋白小泡比年轻母马更常见。在本研究中，多星体似乎与细胞老化有关，而肌动蛋白小泡与卵母细胞供体的老化有关。
