环3',5'-腺苷酸和环3',5'-鸟苷酸对人白细胞迁移及迁移抑制因子（LIF）活性的体外调节作用

In vitro modulation of human leucocyte migration and migration inhibitory factor (LIF) activity by cyclic 3',5'-AMP and cyclic 3',5'-GMP.

作者信息

Bendtzen K, Palit J

出版信息

Acta Pathol Microbiol Scand C. 1977 Feb;85(1):78-80. doi: 10.1111/j.1699-0463.1977.tb03614.x.

DOI:10.1111/j.1699-0463.1977.tb03614.x

PMID:190856

Abstract

The effects of cyclic 3', 5'-AMP (cAMP) and cyclic 3', 5'-GMP (cGMP) on the in vitro migration of human peripheral blood leucocytes under agarose and on the activity of leucocyte migration inhibitory factor (LIF) was studied. Leucocyte migration was not influenced by dibutyryl cAMP, while the dibutyryl derivative of cGMP significantly stimulated cell migration (1 x 10(-5)M). LIF-treated leucocytes partially escaped migration inhibition in the presence of dibutyryl cAMP (greater than or equal to 1 x 10(-6)M), while dibutyryl cGMP was inefficient. If the parent compounds cAMP and cGMP were tested, almost similar results would be obtained, although at higher concentrations of the drugs. These results represent initial experiments with a view to investigating the possible role of cyclic nucleotides in the expression of LIF activity.

摘要

研究了环3'，5'-腺苷酸（cAMP）和环3'，5'-鸟苷酸（cGMP）对人外周血白细胞在琼脂糖下体外迁移以及白细胞迁移抑制因子（LIF）活性的影响。二丁酰cAMP对白细胞迁移没有影响，而cGMP的二丁酰衍生物显著刺激细胞迁移（1×10⁻⁵M）。在存在二丁酰cAMP（大于或等于1×10⁻⁶M）的情况下，经LIF处理的白细胞部分逃脱了迁移抑制，而二丁酰cGMP则无效。如果测试母体化合物cAMP和cGMP，尽管药物浓度较高，但会得到几乎相似的结果。这些结果是为了研究环核苷酸在LIF活性表达中可能的作用而进行的初步实验。