Dwivedi Y, Rastogi R, Sharma S K, Mehrotra R, Garg N K, Dhawan B N
ICMR Centre for Advanced Pharmacological Research on Traditional Remedies, King George's Medical College, Lucknow, India.
Pharmacol Res. 1991 May;23(4):399-407. doi: 10.1016/1043-6618(91)90054-2.
Monocrotaline, a pyrrolizidine alkaloid, caused changes in most of the biochemical parameters in rats 12 days after a single dose of 120 mg/kg. These included significantly increased activities of hepatic succinate dehydrogenase, acid ribonuclease, acid phosphatase, gammaglutamyl transpeptidase and 5'-nucleotidase and decreased in the activities of glucose-6-phosphatase and cytochrome P450. The levels of DNA, RNA and glycogen in liver and albumin and protein in serum decreased while serum bilirubin increased. The histopathological changes in liver were characterized by diffused hepatocyte alterations in the form of ballooning, granular cytoplasm, indistinct cell outlines, nuclear changes, focal necrosis, and vascular damage. When picroliv, a standardized iridoid glycoside fraction of Picrorhiza kurroa, was administered orally in a dose of 25 mg/kg simultaneously with monocrotaline, alterations in most of the biochemical parameters along with the histopathological changes in liver caused by monocrotaline were prevented.
单剂量120mg/kg的野百合碱（一种吡咯里西啶生物碱）在给药12天后会引起大鼠体内大多数生化参数的变化。这些变化包括肝脏琥珀酸脱氢酶、酸性核糖核酸酶、酸性磷酸酶、γ-谷氨酰转肽酶和5'-核苷酸酶的活性显著增加，而葡萄糖-6-磷酸酶和细胞色素P450的活性降低。肝脏中的DNA、RNA和糖原水平以及血清中的白蛋白和蛋白质水平下降，而血清胆红素增加。肝脏的组织病理学变化特征为弥漫性肝细胞改变，表现为气球样变、细胞质颗粒状、细胞轮廓不清、核变化、局灶性坏死和血管损伤。当与野百合碱同时口服给予25mg/kg剂量的苦味叶下珠标准化环烯醚萜苷组分（苦味素）时，可预防野百合碱引起的大多数生化参数变化以及肝脏的组织病理学变化。
