Galinier Richard, Roger Emmanuel, Sautiere Pierre-Eric, Aumelas Andre, Banaigs Bernard, Mitta Guillaume
Laboratoire de Biologie et d'Ecologie Tropicale et Méditerranéenne, CNRS-UPVD-EPHE, Perpignan, France.
J Pept Sci. 2009 Jan;15(1):48-55. doi: 10.1002/psc.1101.
We report here the screening of five marine invertebrate species from two taxa (tunicates and echinoderms) for the presence of cationic antimicrobial peptides (AMP) in defence cells (hemocytes). Antimicrobial activities were detected only in the two tunicates Microcosmus sabatieri and Halocynthia papillosa. In addition, we report the isolation and characterization of two novel peptides from H. papillosa hemocytes. These molecules display antibacterial activity against Gram-positive and Gram-negative bacteria. Complete peptide characterization was obtained by a combination of Edman degradation and mass spectrometry. The mature molecules, named halocyntin and papillosin, comprise 26 and 34 amino acid residues, respectively. Their primary structure display no significant similarities with previously described AMP.
我们在此报告了对来自两个分类群（被囊动物和棘皮动物）的五种海洋无脊椎动物物种的防御细胞（血细胞）中阳离子抗菌肽（AMP）的筛选情况。仅在两种被囊动物萨氏小宇宙（Microcosmus sabatieri）和乳头海鞘（Halocynthia papillosa）中检测到抗菌活性。此外，我们报告了从乳头海鞘血细胞中分离和鉴定出两种新型肽。这些分子对革兰氏阳性菌和革兰氏阴性菌均具有抗菌活性。通过埃德曼降解法和质谱法相结合完成了肽的完整表征。成熟分子分别命名为海鞘素（halocyntin）和乳头海鞘素（papillosin），分别由26个和34个氨基酸残基组成。它们的一级结构与先前描述的AMP没有明显相似性。
