El-Sagheer Afaf H, Brown Tom
School of Chemistry, University of Southampton, Highfield Southampton, UK.
Curr Protoc Nucleic Acid Chem. 2008 Dec;Chapter 4:Unit 4.33. doi: 10.1002/0471142700.nc0433s35.
The Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reaction has been used to synthesize cyclic mini-DNA duplexes. The reaction is carried out on 5'-alkyne-3'-azide-labeled hairpin loop oligonucleotides and proceeds in high yield under mild conditions in as little as 5 min. The resultant duplexes have very high thermal stability and their CD spectra are characteristic of normal B-DNA.
铜（I）催化的炔烃-叠氮化物环加成（CuAAC）反应已被用于合成环状微型DNA双链体。该反应在5'-炔烃-3'-叠氮化物标记的发夹环寡核苷酸上进行，在温和条件下只需5分钟就能高产率地进行。所得的双链体具有非常高的热稳定性，其圆二色光谱具有正常B-DNA的特征。
