Carlson Mark W, Alt-Holland Addy, Egles Christophe, Garlick Jonathan A
School of Dental Medicine, Tufts University, Boston, Massachusetts, USA.
Curr Protoc Cell Biol. 2008 Dec;Chapter 19:Unit 19.9. doi: 10.1002/0471143030.cb1909s41.
Over the last decade, the development of in vitro, human, three-dimensional (3D) tissue models, known as human skin equivalents (HSEs), has furthered understanding of epidermal cell biology and provided novel experimental systems. Signaling pathways that mediate the linkage between growth and differentiation function optimally when cells are spatially organized to display the architectural features seen in vivo, but are uncoupled and lost in two-dimensional culture systems. HSEs consist of a stratified squamous epithelium grown at an air-liquid interface on a collagen matrix populated with dermal fibroblasts. These 3D tissues demonstrate in vivo-like epithelial differentiation and morphology, and rates of cell division, similar to those found in human skin. This unit describes fabrication of HSEs, allowing the generation of human tissues that mimic the morphology, differentiation, and growth of human skin, as well as disease processes of cancer and wound re-epithelialization, providing powerful new tools for the study of diseases in humans.
在过去十年中，被称为人皮肤等效物（HSEs）的体外人体三维（3D）组织模型的发展，加深了对表皮细胞生物学的理解，并提供了新的实验系统。当细胞在空间上组织起来以展现体内所见的结构特征时，介导生长与分化之间联系的信号通路能最佳发挥作用，但在二维培养系统中这些信号通路会解偶联并丧失功能。HSEs由在胶原基质上的气液界面生长的复层鳞状上皮组成，该胶原基质中含有真皮成纤维细胞。这些3D组织展现出类似体内的上皮分化和形态，以及与人类皮肤中相似的细胞分裂速率。本单元描述了HSEs的制备方法，能够生成模拟人类皮肤形态、分化、生长以及癌症疾病过程和伤口再上皮化的人体组织，为人类疾病研究提供了强大的新工具。
