Reuter Christian, Walles Heike, Groeber Florian
Tissue Engineering and Regenerative Medicine, University Hospital Wuerzburg, Wuerzburg, Germany.
Translational Center Wuerzburg 'Regenerative Therapies in Oncology and Musculoskeletal Diseases', Wuerzburg Branch of the Fraunhofer-Institute Interfacial Engineering and Biotechnology, IGB, Roentgenring 11, 97070, Wuerzburg, Germany.
Methods Mol Biol. 2017;1612:191-198. doi: 10.1007/978-1-4939-7021-6_14.
In vitro test systems are a promising alternative to animal models. Due to the use of human cells in a three-dimensional arrangement that allows cell-cell or cell-matrix interactions these models may be more predictive for the human situation compared to animal models or two-dimensional cell culture systems. Especially for dermatological research, skin models such as epidermal or full-thickness skin equivalents (FTSE) are used for different applications. Although epidermal models provide highly standardized conditions for risk assessment, FTSE facilitate a cellular crosstalk between the dermal and epidermal layer and thus can be used as more complex models for the investigation of processes such as wound healing, skin development, or infectious diseases. In this chapter, we describe the generation and culture of an FTSE, based on a collagen type I matrix and provide troubleshooting tips for commonly encountered technical problems.
体外测试系统是动物模型的一种有前途的替代方案。由于使用了三维排列的人类细胞，允许细胞间或细胞与基质的相互作用，与动物模型或二维细胞培养系统相比，这些模型对人类情况可能更具预测性。特别是在皮肤病学研究中，表皮或全层皮肤等效物（FTSE）等皮肤模型被用于不同的应用。尽管表皮模型为风险评估提供了高度标准化的条件，但FTSE促进了真皮和表皮层之间的细胞间相互作用，因此可以用作更复杂的模型来研究伤口愈合、皮肤发育或传染病等过程。在本章中，我们描述了基于I型胶原基质的FTSE的生成和培养，并为常见的技术问题提供故障排除提示。
