Egles Christophe, Garlick Jonathan A, Shamis Yulia
Division of Cancer Biology and Tissue Engineering, Department of Oral and Maxillofacial Pathology, School of Dental Medicine, Sackler Graduate School and School of Engineering, Tufts University, Boston, MA, USA.
Methods Mol Biol. 2010;585:345-59. doi: 10.1007/978-1-60761-380-0_24.
Human skin equivalents (HSEs) are in vitro tissues in which a fully differentiated, stratified squamous epithelium is grown at an air-liquid interface on a Type I collagen gel harboring human dermal fibroblasts. HSEs now provide experimental human tissue models to study factors that direct re-epithelialization and epithelial-mesenchymal cross-talk following wounding. This chapter describes the fabrication of HSEs from human keratinocytes and fibroblasts and how HSEs can be modified to characterize the response of the human epithelium during wound repair. The protocols outlined first describe techniques for the generation of human tissues that closely approximate the architectural features, differentiation, and growth of human skin. This will be followed by a description of a protocol that enables HSEs to be adapted to monitor their response following wounding. These engineered human tissues provide powerful tools to study biological process in tissues that mimic the healing of human skin and of the epithelial tissue.
人皮肤替代物（HSEs）是一种体外组织，在含有人类真皮成纤维细胞的I型胶原凝胶上的气液界面生长出完全分化的复层鳞状上皮。HSEs现在提供了实验性人类组织模型，用于研究伤口愈合后指导再上皮化和上皮-间充质相互作用的因素。本章描述了从人类角质形成细胞和成纤维细胞制备HSEs的方法，以及如何对HSEs进行修饰以表征伤口修复过程中人类上皮的反应。首先概述的方案描述了生成与人类皮肤的结构特征、分化和生长非常相似的人类组织的技术。接下来将描述一个方案，该方案使HSEs能够适应监测其受伤后的反应。这些工程化的人类组织为研究模拟人类皮肤和上皮组织愈合的组织中的生物学过程提供了强大的工具。
