Baek Jean-Ha, Zheng Yiwen, Darlington Cynthia L, Smith Paul F
Department of Pharmacology and Toxicology, School of Medical Sciences, University of Otago, Dunedin, New Zealand.
Acta Otolaryngol. 2008 Sep;128(9):961-7. doi: 10.1080/00016480701796944.
This evidence suggests that both CB1 and CB2 receptors are important in the control of balance and hearing.
Although the cannabinoid CB1 receptor has been identified in the brainstem vestibular and cochlear nuclei, the existence of the second cannabinoid receptor subtype, the CB2 receptor, has been more controversial. The aim of this study was to determine whether or not CB2 receptors are expressed in the vestibular and cochlear nuclei.
Data were obtained from four young male Wistar rats In analyzing the presence of CB2 receptors in the vestibular and cochlear nuclei, the immunohistochemical complex was visualized by exposure to diaminobenzidine for 20 min. Positive immunoreactivity to CB2 was expressed as brown staining in the cytoplasm, nucleus, nuclear membrane and cell membrane.
We confirmed the existence of the CB2 receptor in the vestibular and cochlear nuclei in the brainstem of Wistar rats.
该证据表明CB1和CB2受体在平衡和听力控制中均起重要作用。
尽管已在脑干前庭和耳蜗核中鉴定出大麻素CB1受体，但第二种大麻素受体亚型CB2受体的存在一直存在更多争议。本研究的目的是确定CB2受体是否在前庭和耳蜗核中表达。
数据取自4只年轻雄性Wistar大鼠。在分析前庭和耳蜗核中CB2受体的存在时，通过暴露于二氨基联苯胺20分钟使免疫组织化学复合物显色。对CB2的阳性免疫反应表现为细胞质、细胞核、核膜和细胞膜中的棕色染色。
我们证实了Wistar大鼠脑干的前庭和耳蜗核中存在CB2受体。
