Hadibarata Tony, Tachibana Sanro, Itoh Kazutaka
Department of Applied Bioscience, Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan.
Pak J Biol Sci. 2007 Aug 1;10(15):2535-43. doi: 10.3923/pjbs.2007.2535.2543.
Microbial degradation of Phenanthrene with several fungi screened from nature was conducted to select fungi for the bioremediation ofPhenanthrene. Thrichoderma sp. S019, a fungus collected from soil, had the highest rate of degradation on the agar medium containing Phenanthrene. Maximal degradation (72%) was obtained when Trichoderma sp. S019 was incubated for 30 days after the addition of 0.1 mM of Phenanthrene to the liquid medium. Furthermore, the degradation of Phenanthrene was affected by the addition of a carbon source, the addition of a nitrogen source and agitation. Also, 1,2-Dioxygenase and 2,3-Dioxygenase were produced by Trichoderma sp. S019 in a liquid medium. These enzymes play an important role in the metabolism of substrates, revealing a high stereoselectivity for initial dioxygenase and enzymatic hydration since the K-region of phenanthrene was the major site of metabolism. Phenanthrene was indeed degraded by Trichoderma sp. S019 because 1-Hydroxy-2-naphthoic acid, Salicyaldehyde, Salicylic acid and Catechol, considered to be the intermediates in the bioremediation of Phenanthrene, were detected among the reaction products.
对从自然界筛选出的几种真菌进行菲的微生物降解实验，以筛选用于菲生物修复的真菌。从土壤中分离得到的木霉属真菌Trichoderma sp. S019在含菲的琼脂培养基上具有最高的降解率。在液体培养基中添加0.1 mM菲后，将Trichoderma sp. S019培养30天，可获得最大降解率（72%）。此外，碳源添加、氮源添加和振荡对菲的降解有影响。而且，Trichoderma sp. S019在液体培养基中产生了1,2 - 双加氧酶和2,3 - 双加氧酶。这些酶在底物代谢中起重要作用，由于菲的K区域是主要代谢位点，因此对初始双加氧酶和酶促水合反应具有高立体选择性。在反应产物中检测到了1 - 羟基 - 2 - 萘甲酸、水杨醛、水杨酸和邻苯二酚，它们被认为是菲生物修复过程中的中间产物，这表明菲确实被Trichoderma sp. S019降解了。
