Saikia Nirmali, Gopal Madhuban
Division of Agricultural Chemicals, Indian Agricultural Research Institute, New Delhi-110012, India.
J Agric Food Chem. 2004 Mar 10;52(5):1220-3. doi: 10.1021/jf0349580.
Five fungal species, namely, Trichoderma viride strain 5-2, T. viride strain 2211, Aspergillus niger, A. terricola, and Phanerochaete chrysoporium were screened for degradation study of beta-cyfluthrin. Each fungal species was allowed to grow in Czapek dox medium containing beta-cyfluthrin (5 mg/mL) as the major carbon source of the medium. The highest degradation of beta-cyfluthrin was observed by T. viride 5-2 (T(1/2) = 7.07 days), followed by T.viride 2211 (T(1/2) = 10.66 days). The degradation of beta-cyfluthrin followed first-order kinetics with a fast degradation rate during first 7 days of growth of the fungi. In the case of T. viride strain 5-2, five degradation products were isolated after 20 days of growth of the fungi, out of which three products were identified as alpha-cyano-4-fluorobenzyl-3- (2,2-dichlorovinyl)-2,2-dimethyl cyclopropane carboxylate, alpha-cyano-4-fluoro-3-phenoxy benzyl alcohol, and 3(2,2-dichlorovinyl)-2,2-dimethyl cyclopropanoic acid.
筛选了5种真菌,即绿色木霉5-2菌株、绿色木霉2211菌株、黑曲霉、土曲霉和黄孢原毛平革菌,用于高效氟氯氰菊酯的降解研究。每种真菌都在含有高效氟氯氰菊酯(5毫克/毫升)作为主要碳源的查氏培养基中生长。绿色木霉5-2对高效氟氯氰菊酯的降解率最高(半衰期=7.07天),其次是绿色木霉2211(半衰期=10.66天)。高效氟氯氰菊酯的降解符合一级动力学,在真菌生长的前7天降解速率较快。在绿色木霉5-2菌株的情况下,真菌生长20天后分离出5种降解产物,其中3种产物被鉴定为α-氰基-4-氟苄基-3-(2,2-二氯乙烯基)-2,2-二甲基环丙烷羧酸酯、α-氰基-4-氟-3-苯氧基苄醇和3(2,2-二氯乙烯基)-2,2-二甲基环丙酸。