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利用叶绿体微卫星标记评估伊朗野生和栽培葡萄的遗传多样性。

Chloroplast microsatellites markers to assess genetic diversity in wild and cultivated grapevines of Iran.

作者信息

Doulaty Baneh H, Mohammadi S A, Labra M, Nazemieh A, De Mattia F, Mardi M

机构信息

University of Tabriz, Tabriz 51666, Iran.

出版信息

Pak J Biol Sci. 2007 Jun 1;10(11):1855-9. doi: 10.3923/pjbs.2007.1855.1859.

Abstract

To assess the haplotype diversity and genetic relationship between them, A set of 69 Iranian cultivated accessions, six European cultivars and an accession of Vitis labrusca along with 63 wild grapevine individuals were studied using chloroplast microsatellite markers. Results showed that among analyzed cpssr loci only ccmp 3 and ccmp10 were polymorphic within cultivars and only ccmp3 was polymorphic in wild grape individuals. The size variants of both loci combine in a total of 4 different haplotypes. All the 4 haplotype are displayed in the cultivars while only 2 are presented in wild grapes. Sultani or keshmeshi Bidane cultivar has the haplotype III that there is not this haplotype among the wild grapes of studied regions. Concerning to existence of both haplotypes I and II in the number of Iranian cultivated and wild grapes, it is possible to consider that the wild grapes are ancestor of some of our native cultivars.

摘要

为了评估它们之间的单倍型多样性和遗传关系,利用叶绿体微卫星标记对一组69个伊朗栽培种、6个欧洲品种、1个美洲葡萄品种以及63个野生葡萄个体进行了研究。结果表明,在所分析的叶绿体简单序列重复(cpssr)位点中,只有ccmp 3和ccmp10在栽培品种中具有多态性,而只有ccmp3在野生葡萄个体中具有多态性。这两个位点的大小变异总共组合成4种不同的单倍型。所有4种单倍型都出现在栽培品种中,而野生葡萄中只出现了2种。苏丹尼或凯什梅希·比丹品种具有单倍型III,在所研究地区的野生葡萄中没有这种单倍型。关于单倍型I和II在伊朗栽培葡萄和野生葡萄中的存在情况,可以认为野生葡萄是我们一些本土栽培品种的祖先。

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