Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Engineering Research Center of Chinese Medicine Resources, Ministry of Education, Beijing 100193, China.
Int J Mol Sci. 2019 Aug 19;20(16):4040. doi: 10.3390/ijms20164040.
is an important medicinal and edible plant with several pharmacologically active volatile oils. However, identifying from var. and which exhibit similar morphological characteristics to , is difficult. The main goal of this study, therefore, is to mine genetic resources and improve molecular methods that could be used to distinguish these species. A total of eight complete chloroplasts (cp) genomes of these species which were collected from the main producing areas in China were determined to be 163,608-164,069 bp in size. All genomes displayed a typical quadripartite structure with a pair of inverted repeat (IR) regions (29,820-29,959 bp) that separated a large single copy (LSC) region (88,680-88,857 bp) from a small single copy (SSC) region (15,288-15,369 bp). Each genome encodes 113 different genes with 79 protein-coding genes, 30 tRNA genes, and four rRNA genes. More than 150 SSRs were identified in the entire cp genomes of these three species. The Sanger sequencing results based on 32 samples indicated that five highly divergent regions screened from cp genomes could not be used to distinguish species. Phylogenetic analysis showed that the cp genomes could not only accurately identify species, but also provide a solid foundation for the establishment of phylogenetic relationships of species. The availability of cp genome resources and the comparative analysis is beneficial for species authentication and phylogenetic analysis in .
是一种重要的药用和食用植物，具有几种具有药理活性的挥发油。然而，从形态特征与 相似的 和 中鉴定 却很困难。因此，本研究的主要目的是挖掘遗传资源并改进分子方法，以区分这些物种。从中国主要产区收集的这 3 种植物的 8 个完整叶绿体（cp）基因组，大小为 163608-164069bp。所有基因组均显示出典型的四部分结构，一对反向重复（IR）区（29820-29959bp）将大单一拷贝（LSC）区（88680-88857bp）与小单一拷贝（SSC）区（15288-15369bp）分隔开。每个基因组编码 113 个不同的基因，其中 79 个蛋白编码基因、30 个 tRNA 基因和 4 个 rRNA 基因。在这三个物种的整个 cp 基因组中鉴定出 150 多个 SSR。基于 32 个样本的 Sanger 测序结果表明，从 cp 基因组中筛选出的五个高度分化区域不能用于区分 物种。系统发育分析表明，cp 基因组不仅可以准确鉴定 物种，还为 物种的系统发育关系建立提供了坚实的基础。cp 基因组资源的可用性和比较分析有利于 的物种鉴定和系统发育分析。
