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速灭磷(一种布基纳法索农业中使用的有机磷杀虫剂)经驯化的本地细菌进行厌氧生物降解。

Anaerobic biodegradation of sumithion an organophosphorus insecticide used in Burkina Faso agriculture by acclimatized indigenous bacteria.

作者信息

Savadogo Paul W, Savadogo Aly, Ouattara Aboubacar S, Sedogo Michel P, Traoré S

机构信息

Laboratoire Sol Eau Plante, Institut de l'Environnement et de Recherches Agricoles.

出版信息

Pak J Biol Sci. 2007 Jun 1;10(11):1896-905. doi: 10.3923/pjbs.2007.1896.1905.

DOI:10.3923/pjbs.2007.1896.1905
PMID:19086557
Abstract

Biodegradation of Sumithion (o,o-dimethyl o-(3-methyl-4-nitrophenyl) phosphorothioate) was tested using microorganisms isolated from mud under controlled conditions. Results showed that after 2 years of acclimatation, the indigenous microorganisms can biodegrade sumithion under anaerobic conditions. This biodegradation lead to methane production. Biodegradation kinetics monitored by high performance liquid chromatography and gas chromatography showed that the acclimated consortium is able to carry out the biodegradation of 20, 50 and 100 mg L(-1) of Sumithion, respectively in 15, 25 and 45 days. The end products of sumithion biodegradation were gases. Such as CO2, N2, CH4 and H2S. Isolation and partial characterization of a strain able to degrade Sumithion were realized. This strain named SY can be easily produced and used for the bioremediation of waters and soils contaminated by Sumithion.

摘要

使用从泥浆中分离出的微生物,在可控条件下对杀螟硫磷(o,o-二甲基 o-(3-甲基-4-硝基苯基)硫代磷酸酯)的生物降解进行了测试。结果表明,经过2年的驯化,本地微生物能够在厌氧条件下对杀螟硫磷进行生物降解。这种生物降解导致了甲烷的产生。通过高效液相色谱和气相色谱监测的生物降解动力学表明,驯化后的菌群能够分别在15天、25天和45天内对20、50和100 mg L(-1)的杀螟硫磷进行生物降解。杀螟硫磷生物降解的最终产物为气体,如二氧化碳、氮气、甲烷和硫化氢。实现了一株能够降解杀螟硫磷的菌株的分离和部分特性鉴定。该菌株命名为SY,易于培养,可用于被杀螟硫磷污染的水体和土壤的生物修复。

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