Mohabatkar Hassan, Mohammadzadegan Reza
Department of Biology, College of Sciences, Shiraz University, Shiraz 71454, Iran.
Pak J Biol Sci. 2007 Dec 1;10(23):4295-8. doi: 10.3923/pjbs.2007.4295.4298.
In the present study, T-cell epitopes of gp120 of an Iranian isolate have been compared to different subtypes of HIV-1. At first, the amino acid sequences of gp120 were fetched from data banks. Then T-cell epitopes, disulfide bonding states, protein kinase C phosphorylation sites, cAMP-dependent protein kinase phosphorylation sites, casein kinase II phosphorylation sites, N-myristoylation sites and amidation sites were predicted using different soft wares. According to this computational analysis 6 good disulfide binding states in Iranian gp120 were predicted. From the viewpoint of cAMP-dependent protein kinase phosphorylation site (1 site) Iranian isolate was similar to clades B and F. Like subtype C 1 amidation site was predicted in the Iranian subtype. In the Iranian isolate 7 sites protein kinase C phosphorylation sites and 4 N-myristoylation sites were predicted. Since the number of individuals infected with HIV-1 in Iran, like many other countries is increasing, study of similarities and differences between the Iranian samples and different clades of HIV-1 can help us in identification of the origin and understanding the changes in the virus.
在本研究中，已将一种伊朗分离株的gp120的T细胞表位与HIV-1的不同亚型进行了比较。首先，从数据库中获取gp120的氨基酸序列。然后使用不同软件预测T细胞表位、二硫键结合状态、蛋白激酶C磷酸化位点、环磷酸腺苷依赖性蛋白激酶磷酸化位点、酪蛋白激酶II磷酸化位点、N-肉豆蔻酰化位点和酰胺化位点。根据这种计算分析，预测出伊朗gp120中有6种良好的二硫键结合状态。从环磷酸腺苷依赖性蛋白激酶磷酸化位点（1个位点）的角度来看，伊朗分离株与B和F分支相似。与C亚型一样，在伊朗亚型中预测到1个酰胺化位点。在伊朗分离株中，预测到7个蛋白激酶C磷酸化位点和4个N-肉豆蔻酰化位点。由于伊朗与许多其他国家一样，感染HIV-1的人数正在增加，研究伊朗样本与HIV-1不同分支之间的异同有助于我们识别病毒的起源并了解其变化。
