正交设计在怀山药组织培养中的应用

[Application of orthogonal design in tissue culture of Dioscorea opposite].

作者信息

Xue Jian-ping, Zhao Xiao-quan, Zhang Ai-min, Huang Yue-qin

机构信息

Department of Biology, Huaibei Coal Industry Teachers' College, Anhui Key Laboratory of Plant Resources and Biology, Huaibei 235000, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2008 Aug;33(16):1952-5.

PMID:19086626

Abstract

OBJECTIVE

To study the effects of different factors on buds and microtuber. These factors included plant growth substances and sucrose.

METHOD

stems were selected as explants. The effects of three kinds of factors were studied by orthogonal design method including sucrose, 6-BA, NAA on the buds and microtuber induction. The data were analyzed with range analysis and vadance analysis. RESULT AND CONDUSION: The optimal media to induce many buds from stems were MS + 6-BA 1 mg x L(-1) + NAA 1 mg x L(-1) + sucrose 3%, the effect of the three factors was in sequence of sucrose >6-BA > NAA. The optimal media to induce microtuber from stems were MS+6-BA 1.5 mg x V1 +NAA 1.5 mg x L(-1) + sucrose 5%, the effect of the three factors was in sequence of sucrose >6-BA > NAA.

摘要

目的

研究不同因素对芽和微型块茎的影响。这些因素包括植物生长物质和蔗糖。

方法

选取茎段作为外植体。采用正交设计法研究蔗糖、6-苄氨基腺嘌呤（6-BA）、萘乙酸（NAA）三种因素对芽和微型块茎诱导的影响。数据采用极差分析和方差分析。结果与结论：诱导茎段产生多个芽的最佳培养基为MS + 6-BA 1 mg·L⁻¹ + NAA 1 mg·L⁻¹ + 蔗糖3%，三个因素的影响顺序为蔗糖＞6-BA＞NAA。诱导茎段产生微型块茎的最佳培养基为MS + 6-BA 1.5 mg·L⁻¹ + NAA 1.5 mg·L⁻¹ + 蔗糖5%，三个因素的影响顺序为蔗糖＞6-BA＞NAA。