Chen Qingbing, Gao Kang, Pan Bo, Wang Yaoyao, Chen Lijie, Yu Junjun, Wang Lili, Fan Yongming, Li Haiying, Huang Conglin
College of Architecture, North China University of Water Resources and Electric Power, Zhengzhou 450046, China.
Institute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Plants (Basel). 2024 Aug 28;13(17):2403. doi: 10.3390/plants13172403.
'11-C-2' is a variety of chrysanthemums with high ornamental and tea value, experiencing significant market demand. However, as cultivation areas expand, issues such as viral infection, germplasm degradation, low proliferation coefficient, and slow proliferation rate arise, necessitating the establishment of an efficient in vitro regeneration system. This study, based on the principles of orthogonal experimental design, explored the regeneration system of Chrysanthemum cultivar '11-C-2' using sterile seedlings. The research focused on three key stages: adventitious bud differentiation, rooting culture, and acclimatization-transplantation, employing shoot-bearing stem segments and leaves as explants. The findings indicate that the optimal explant for the Chrysanthemum '11-C-2' sterile seedlings is the shoot-bearing stem segment. The best medium for adventitious bud differentiation was determined to be MS supplemented with 1.5 mg/L 6-BA and 0.5 mg/L NAA. Bud differentiation began on day 17 with a 100% differentiation rate, completing around day 48. The maximum differentiation coefficient reached 87, with an average of 26.67. The adventitious buds were then cultured for rooting in the optimal medium of 1/2 MS supplemented with 0.1 mg/L NAA. Rooting was initiated on day 4 and was completed by day 14, achieving a rooting rate of 97.62%. After a 5-day acclimatization under natural light, the rooted seedlings were transplanted into a growth substrate with a peat-to-vermiculite ratio of 1:2. The plants exhibited optimal growth, with a transplantation survival rate of 100%. The findings provide data support for the efficient large-scale propagation of '11-C-2' and lay the foundation for germplasm preservation and genetic transformation research of tea chrysanthemums.
“11 - C - 2”是一种具有较高观赏价值和茶饮价值的菊花品种，市场需求显著。然而，随着种植面积的扩大，出现了病毒感染、种质退化、增殖系数低和增殖速度慢等问题，因此有必要建立高效的离体再生体系。本研究基于正交试验设计原理，以无菌苗为材料探索菊花品种“11 - C - 2”的再生体系。研究重点关注三个关键阶段：不定芽分化、生根培养和驯化移栽，采用带芽茎段和叶片作为外植体。研究结果表明，菊花“11 - C - 2”无菌苗的最佳外植体是带芽茎段。确定不定芽分化的最佳培养基为添加1.5 mg/L 6 - BA和0.5 mg/L NAA的MS培养基。不定芽分化在第17天开始，分化率达100%，约在第48天完成。最大分化系数达到87，平均为26.67。然后将不定芽在添加0.1 mg/L NAA的1/2 MS最佳培养基中进行生根培养。生根在第4天开始，第14天完成，生根率达97.62%。在自然光下驯化5天后，将生根苗移栽到泥炭与蛭石比例为1:2的生长基质中。植株生长良好，移栽成活率达100%。研究结果为“11 - C - 2”的高效大规模繁殖提供了数据支持，并为茶用菊花的种质保存和遗传转化研究奠定了基础。
