[来自无柄蝉翼藤的抗氧化氧杂蒽酮]

Kang Wen-yi, Li Cai-fang, Song Yan-li

Institute of Natural Products, Henan University, Kaifeng 475004, China.

Zhongguo Zhong Yao Za Zhi. 2008 Aug;33(16):1982-5.

OBJECTIVE

To study the antioxidant constituents from the roots of Securidaca inappendiculata.

METHOD

The bioassay-guided isolation of antioxidant constituents was carried out by the column chromatographic techniques. The combination of IR, MS, NMR and 2D-NMR spectroscopics methods was used to identify their structures.

RESULT

Two new xanthones, 1, 2, 5-trihydroxy-6, 8-dimethoxy-9H-xanthen-9-one(1), 1, 5-dihydroxy-2, 6, 8-trimethoxy-9H-xanthen-9-one (2), along with seven known ones, 3, 8-dihydroxy-1, 4-dimethoxy-9H-xanthen-9-one(3), 4, 6-dihydroxy-1, 5, 7-trimethoxy-9H-xanthen-9-one(4), 7-hydroxy-1, 2, 3, 8-tetramethoxy-9H-xanthen- 9-one(5), 1, 7-dihydroxy-9H-xanthen-9-one(6), 4-hydroxy-3, 7-dimethoxy-9H-xanthen-9-one(7), 1,7-dimethoxy-9H-xanthen-9-one(8) and aucuparin(9), were isolated from the roots of S. inappendiculata.

CONCLUSION

Compounds 1 and 2 were new xanthones, and compound 3 was isolated as a natural product for the first time, and compounds 4 and 6 were isolated for the first time from this genus. The antioxidant activities of all compounds were evaluated by ABTS, FRAP and DPPH assays respectively. Compound 9 showed significant activity by the ABTS and FRAP assays. Compound 1 showed significant activity with IC50 value of 0.31 mg x L(-1) in DPPH assay. Scavenging capacity of all compounds determined by all assays were well correlated between ABTS and FRAP assay (r = 0.9555).

目的

研究无柄紫丹根中的抗氧化成分。

方法

采用柱色谱技术进行生物活性导向的抗氧化成分分离。运用红外光谱、质谱、核磁共振光谱和二维核磁共振光谱方法相结合来鉴定其结构。

结果

从无柄紫丹根中分离得到两个新的氧杂蒽酮，1,2,5 - 三羟基 - 6,8 - 二甲氧基 - 9H - 氧杂蒽 - 9 - 酮（1）、1,5 - 二羟基 - 2,6,8 - 三甲氧基 - 9H - 氧杂蒽 - 9 - 酮（2），以及七个已知的氧杂蒽酮，3,8 - 二羟基 - 1,4 - 二甲氧基 - 9H - 氧杂蒽 - 9 - 酮（3）、4,6 - 二羟基 - 1,5,7 - 三甲氧基 - 9H - 氧杂蒽 - 9 - 酮（4）、7 - 羟基 - 1,2,3,8 - 四甲氧基 - 9H - 氧杂蒽 - 9 - 酮（5）、1,7 - 二羟基 - 9H - 氧杂蒽 - 9 - 酮（6）、4 - 羟基 - 3,7 - 二甲氧基 - 9H - 氧杂蒽 - 9 - 酮（7）、1,7 - 二甲氧基 - 9H - 氧杂蒽 - 9 - 酮（8）和鹰爪豆黄素（9）。

结论

化合物1和2是新的氧杂蒽酮，化合物3首次作为天然产物被分离得到，化合物4和6首次从该属植物中分离得到。分别采用ABTS、FRAP和DPPH法对所有化合物的抗氧化活性进行了评价。化合物9在ABTS和FRAP法中表现出显著活性。化合物1在DPPH法中表现出显著活性，IC50值为0.31 mg·L⁻¹。所有化合物在ABTS和FRAP法中的清除能力测定结果具有良好的相关性（r = 0.9555）。