从红树林内生真菌（编号 ZH19）中分离得到的两种黄烷酮衍生物的结构阐明和 NMR 归属。

Structure elucidation and NMR assignments for two xanthone derivatives from a mangrove endophytic fungus (No. ZH19).

机构信息

College of Chemistry and Ecology Engineering, Guangxi University for Nationalities, Nanning 530006, P. R. China.

出版信息

Magn Reson Chem. 2010 Jan;48(1):80-2. doi: 10.1002/mrc.2539.

PMID:19908227

Abstract

The structure elucidations and complete (1)H and (13)C NMR assignments are reported for two new xanthone derivatives: 1,7-dihydroxy-2-methoxy-3-(3-methylbut-2-enyl)-9H-xanthen-9-one (1) and 1-hydroxy-4,7-dimethoxy-6-(3-oxobutyl)-9H-xanthen-9-one (2). Both of these secondary metabolites were isolated from the fermentation medium of a mangrove endophytic fungus (No. ZH19). High-resolution electron impact mass spectrometry (HREIMS), Fourier transform infrared (FT-IR) absorption spectrometry, and NMR experiments including gCOSY, gHMQC, and gHMBC were used for the determination of the structures and NMR spectral assignments. Preliminary pharmacological test showed that compounds (1) and (2) inhibited KB cells with IC(50) values of 20 and 35 micromol/ml, and KB(V)200 cells with IC(50) values of 30 and 41 micromol/ml, respectively.

摘要

报道了两种新的酮衍生物

1,7-二羟基-2-甲氧基-3-(3-甲基-2-丁烯基)-9H-呫吨-9-酮（1）和 1-羟基-4,7-二甲氧基-6-(3-氧代丁基)-9H-呫吨-9-酮（2）的结构阐明和全（1）H 和（13）C NMR 分配。这两种次生代谢产物均从红树林内生真菌（编号 ZH19）的发酵培养基中分离得到。高分辨率电子碰撞质谱（HREIMS）、傅里叶变换红外（FT-IR）吸收光谱以及包括 gCOSY、gHMQC 和 gHMBC 的 NMR 实验用于确定结构和 NMR 光谱分配。初步药理试验表明，化合物（1）和（2）对 KB 细胞的 IC（50）值分别为 20 和 35 μmol/ml，对 KB（V）200 细胞的 IC（50）值分别为 30 和 41 μmol/ml。