Li Jing-ruo, Li Meng-quan, Bao Jun-tao, Li Jian-zhang
Department of Breast Disease Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Zhonghua Yi Xue Za Zhi. 2008 Aug 19;88(32):2278-80.
To investigate the expression of metastasis-associated gene 1 (MTA1) and matrix metalloproteinase 9 (MMP-9) gene in breast carcinoma and the relationship between the expression of these genes and the invasion of breast cancer.
Fluorescence quantitative PCR technique was used to detect the mRNA expression of MTA1 and MMP-9 gene among 56 human breast cancer samples.
The mRNA expression rate of MTA1 was higher in 83.9% (47/56) of the primary breast cancer tissues compared with the matched normal breast tissues. The mRNA expression of MMP-9 was higher in 85.7% (48/56) of the primary breast cancer tissues compared to the matched normal breast tissues. The over-expression of MTA1 and that of the MMP-9 gene were significantly related with the degree of differentiation, clinical stage, and lymph node metastasis of breast cancer (all P < 0.05). The positive ratio of MTA1 gene is tightly associated with that of MMP-9 gene (P < 0.05).
The united detection of MTA1 and MMP-9 gene expression predicts the invasion and metastasis of breast cancer and supplies evidence for clinical therapy and judgment of prognosis. MTA1 and MMP-9 will become new targets for gene therapy.
探讨转移相关基因1（MTA1）和基质金属蛋白酶9（MMP - 9）基因在乳腺癌中的表达情况以及这些基因表达与乳腺癌侵袭之间的关系。
采用荧光定量PCR技术检测56例人乳腺癌样本中MTA1和MMP - 9基因的mRNA表达。
与配对的正常乳腺组织相比，83.9%（47/56）的原发性乳腺癌组织中MTA1的mRNA表达率更高。与配对的正常乳腺组织相比，85.7%（48/56）的原发性乳腺癌组织中MMP - 9的mRNA表达更高。MTA1的过表达和MMP - 9基因的过表达均与乳腺癌的分化程度、临床分期及淋巴结转移显著相关（均P < 0.05）。MTA1基因的阳性率与MMP - 9基因的阳性率密切相关（P < 0.05）。
联合检测MTA1和MMP - 9基因表达可预测乳腺癌的侵袭和转移，为临床治疗及预后判断提供依据。MTA1和MMP - 9将成为基因治疗的新靶点。
