Sekizawa Akihiko, Purwosunu Yuditiya, Yoshimura Shiho, Nakamura Masamitsu, Shimizu Hanako, Okai Takashi, Rizzo Nicola, Farina Antonio
Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
Reprod Sci. 2009 Apr;16(4):408-13. doi: 10.1177/1933719108328615. Epub 2008 Dec 15.
To assess the role of placental protein 13 (PP13) in the pathogenesis of preeclampsia, the messenger RNA expression of PP13 was investigated in the trophoblasts from first and third trimester placenta. First, syncytiotrophoblasts and extravillous trophoblasts were separated from frozen section of third trimester placenta obtained from pregnant women with and without preeclampsia by laser microdissection. The PP13 levels of syncytiotrophoblasts were significantly higher than those of extravillous trophoblasts. The expression levels in syncytiotrophoblasts from the preeclampsia were significantly lower than those from normal. Next, the PP13 expression was assessed in trophoblasts from residual samples of chorionic villus sampling at 11 weeks of gestation, who subsequently developed preeclampsia. The levels in the preeclampsia group were significantly lower than those of normal cases. These findings indicate that an alteration in the PP13-messenger RNA expression in the trophoblasts may be associated with the pathogenesis of preeclampsia.
为评估胎盘蛋白13（PP13）在子痫前期发病机制中的作用，研究了来自孕早期和孕晚期胎盘的滋养层细胞中PP13的信使核糖核酸表达。首先，通过激光显微切割从患有和未患子痫前期的孕妇所获得的孕晚期胎盘冰冻切片中分离出合体滋养层细胞和绒毛外滋养层细胞。合体滋养层细胞的PP13水平显著高于绒毛外滋养层细胞。子痫前期患者的合体滋养层细胞中的表达水平显著低于正常患者。接下来，对妊娠11周时绒毛取样残余样本中随后发生子痫前期的滋养层细胞中的PP13表达进行了评估。子痫前期组的水平显著低于正常病例。这些发现表明，滋养层细胞中PP13信使核糖核酸表达的改变可能与子痫前期的发病机制有关。
