Department of Histology, Embryology and Applied Biology, Division of Prenatal Medicine, Via Belmeloro 8, 40126 Bologna, Italy.
Prenat Diagn. 2009 Nov;29(11):1038-44. doi: 10.1002/pd.2344.
To determine the gene expression profile in chorionic villous samples (CVS) of women destined to develop preeclampsia.
cDNA microarray technology was employed. Ten singleton fetuses of women who subsequently developed preeclampsia where compared with a pool of 50 controls. The mRNA expression of some of the genes previously found to be up- or down-regulated were validated by RT-PCR in peripheral blood from 23 pregnant women at term affected with preeclampsia and 23 controls.
Altered expression was found among several genes including those involved in invasion of human trophoblasts (Titin), in inflammatory stress (Lactotransferrin), endothelial aberration (Claudin 6), angiogenesis (Vasohibin 1), blood pressure control (Adducin 1). Also the peripheral blood from preeclampsia patients showed significant differences for all the genes studied.
CVS show an aberrant gene profile prior to preeclampsia onset that may be predictive of the disease.
确定子痫前期患者绒毛样本（CVS）中的基因表达谱。
采用 cDNA 微阵列技术。将随后发生子痫前期的 10 名单胎胎儿与 50 名对照组进行比较。通过 RT-PCR 在 23 名患有子痫前期的足月孕妇和 23 名对照者的外周血中验证了一些先前发现的上调或下调的基因的 mRNA 表达。
在包括参与人滋养层浸润的基因（Titin）、炎症应激（Lactotransferrin）、血管内皮异常（Claudin 6）、血管生成（Vasohibin 1）、血压控制（Adducin 1）在内的几个基因中发现了改变的表达。子痫前期患者的外周血也显示出所有研究基因的显著差异。
CVS 在子痫前期发作前表现出异常的基因谱，这可能是疾病的预测指标。
