Celestino J J H, Bruno J B, Lima-Verde I B, Matos M H T, Saraiva M V A, Chaves R N, Martins F S, Lima L F, Name K P O, Campello C C, Silva J R V, Báo S N, Figueiredo J R
Faculty of Veterinary Medicine, LAMOFOPA, PPGCV, State University of Ceara, Fortaleza-CE Brazil.
Reprod Sci. 2009 Mar;16(3):239-46. doi: 10.1177/1933719108325756. Epub 2008 Dec 15.
We investigated the effects of epidermal growth factor on the survival and growth of caprine preantral follicles. Ovarian fragments were cultured for 1 and 7 days in enriched minimal essential medium with epidermal growth factor (0, 1, 10, 50, 100, or 200 ng/mL). Non-cultured and cultured tissues were processed for histological and ultrastructural studies. Results showed that after 7 days, the epidermal growth factor (1 and 10 ng/mL) maintained the percentage of normal follicles similar to control. An increase in the percentage of primary follicles was observed with 1, 10, and 50 ng/mL of epidermal growth factor compared to enriched minimal essential medium. Ultrastructural studies confirmed follicular integrity after 7 days in epidermal growth factor (1 and 10 ng/mL). In conclusion, the low concentrations of epidermal growth factor maintain caprine follicular viability and promote the transition from primordial to primary follicles.
我们研究了表皮生长因子对山羊腔前卵泡存活和生长的影响。将卵巢片段在添加表皮生长因子（0、1、10、50、100或200 ng/mL）的富集基础培养基中培养1天和7天。对未培养和培养后的组织进行组织学和超微结构研究。结果显示，7天后，表皮生长因子（1和10 ng/mL）使正常卵泡的百分比维持在与对照组相似的水平。与富集基础培养基相比，1、10和50 ng/mL的表皮生长因子使初级卵泡的百分比增加。超微结构研究证实，在添加表皮生长因子（1和10 ng/mL）培养7天后卵泡结构完整。总之，低浓度的表皮生长因子可维持山羊卵泡的活力，并促进原始卵泡向初级卵泡的转变。
