Wang Wenzhao, Morohoshi Tomohiro, Ikeda Tsukasa, Chen Liang
Department of Material and Environmental Chemistry, Utsunomiya University, Utsunomiya, Tochigi, Japan.
Acta Biochim Biophys Sin (Shanghai). 2008 Dec;40(12):1023-8. doi: 10.1111/j.1745-7270.2008.00490.x.
In the present study, we investigated the inhibition of the Lux quorum-sensing system by N-acyl cyclopentylamine (Cn-CPA). The Lux quorum-sensing system regulates luminescence gene expression in Vibrio fischeri. We have already reported on the synthesis of Cn-CPA and their abilities as inhibitors of the quorum-sensing systems in Pseudomonas aeruginosa and Serratia marcescens. In the case of Pseudomonas aeruginosa (Las and Rhl quorum-sensing system) and Serratia marcescens (Spn quorum-sensing system), specific Cn-CPA with a particular acyl chain length showed the strongest inhibitory effect. In the case of the Lux quorum-sensing system, it was found that several kinds of Cn-CPA with a range from C5 to C10 showed similar strong inhibitory effects. Moreover, the inhibitory effect of Cn-CPA on the Lux quorum-sensing system was stronger than that of halogenated furanone, a natural quorum-sensing inhibitor.
在本研究中，我们研究了N-酰基环戊胺（Cn-CPA）对Lux群体感应系统的抑制作用。Lux群体感应系统调节费氏弧菌中发光基因的表达。我们已经报道了Cn-CPA的合成及其作为铜绿假单胞菌和粘质沙雷氏菌群体感应系统抑制剂的能力。在铜绿假单胞菌（Las和Rhl群体感应系统）和粘质沙雷氏菌（Spn群体感应系统）的情况下，具有特定酰基链长度的特定Cn-CPA表现出最强的抑制作用。在Lux群体感应系统的情况下，发现几种碳链长度从C5到C10的Cn-CPA表现出相似的强抑制作用。此外，Cn-CPA对Lux群体感应系统的抑制作用强于天然群体感应抑制剂卤代呋喃酮。
