用于实时监测活细胞中cGMP的新技术。

Novel techniques for real-time monitoring of cGMP in living cells.

作者信息

Nikolaev Viacheslav O, Lohse Martin J

机构信息

Rudolf Virchow Center, University of Würzburg, Germany.

出版信息

Handb Exp Pharmacol. 2009(191):229-43. doi: 10.1007/978-3-540-68964-5_11.

DOI:10.1007/978-3-540-68964-5_11

PMID:19089332

Abstract

Recent developments of biophysical and electrophysiological techniques have enabled researchers to monitor levels of free intracellular cGMP in real-time and in intact living cells. These techniques are based on the use of cGMP sensors, which respond to cGMP with changes in transmembrane ion current or changes in fluorescence. Here, we describe the principles of these techniques, compare them in terms of sensitivity and discuss possible application for current cell biology and physiology.

摘要

生物物理和电生理技术的最新进展使研究人员能够实时监测完整活细胞内游离cGMP的水平。这些技术基于cGMP传感器的使用，该传感器会随着跨膜离子电流的变化或荧光的变化对cGMP做出反应。在这里，我们描述这些技术的原理，在灵敏度方面对它们进行比较，并讨论它们在当前细胞生物学和生理学中的可能应用。