Nikolaev Viacheslav O, Lohse Martin J
Institute of Pharmacology and Toxicology, University of Würzburg, Würzburg, Germany.
Physiology (Bethesda). 2006 Apr;21:86-92. doi: 10.1152/physiol.00057.2005.
cAMP is an important second messenger with a plethora of cellular effects and biological roles. To monitor and visualize cAMP in intact living cells, electrophysiological and fluorescent methods have been developed based on activation of all three types of cAMP effectors: protein kinase A, cyclic nucleotide-gated channels, and exchange protein directly activated by cAMP. In this review, we describe and compare these techniques in terms of their robustness, sensitivity and spatio-temporal resolution.
环磷酸腺苷（cAMP）是一种重要的第二信使，具有众多细胞效应和生物学作用。为了监测和可视化完整活细胞中的cAMP，基于三种类型的cAMP效应器（蛋白激酶A、环核苷酸门控通道和直接由cAMP激活的交换蛋白）的激活，已经开发了电生理和荧光方法。在这篇综述中，我们从稳健性、灵敏度和时空分辨率方面描述并比较了这些技术。
