Shinin Vasily, Gayraud-Morel Barbara, Tajbakhsh Shahragim
Stem Cells and Development, Department of Developmental Biology, Pasteur Institute, Paris, France.
Methods Mol Biol. 2009;482:295-317. doi: 10.1007/978-1-59745-060-7_19.
Stem cells are present in all tissues and organs, and are crucial for normal regulated growth. How the pool size of stem cells and their progeny is regulated to establish the tissue prenatally, then maintain it throughout life, is a key question in biology and medicine. The ability to precisely locate stem and progenitors requires defining lineage progression from stem to differentiated cells, assessing the mode of cell expansion and self-renewal and identifying markers to assess the different cell states within the lineage. We have shown that during lineage progression from a quiescent adult muscle satellite cell to a differentiated myofibre, both symmetric and asymmetric divisions take place. Furthermore, we provide evidence that a sub-population of label retaining satellite cells co-segregate template DNA strands to one daughter cell. These findings provide a means of identifying presumed stem and progenitor cells within the lineage. In addition, asymmetric segregation of template DNA and the cytoplasmic protein Numb provides a landmark to define cell behaviour as self-renewal and differentiation decisions are being executed.
干细胞存在于所有组织和器官中，对正常的生长调节至关重要。在产前如何调节干细胞及其后代的数量以形成组织，然后在一生中维持该组织，是生物学和医学中的关键问题。精确定位干细胞及其祖细胞的能力需要确定从干细胞到分化细胞的谱系进展，评估细胞扩增和自我更新的模式，并识别用于评估谱系内不同细胞状态的标志物。我们已经表明，在从静止的成年肌肉卫星细胞到分化的肌纤维的谱系进展过程中，对称和不对称分裂都会发生。此外，我们提供证据表明，一群标记保留卫星细胞将模板DNA链共分离到一个子细胞中。这些发现提供了一种识别谱系内假定的干细胞和祖细胞的方法。此外，模板DNA和细胞质蛋白Numb的不对称分离提供了一个标志，用于定义在执行自我更新和分化决定时的细胞行为。
