使用回弹式眼压计测量前房充气大鼠的眼压。

Assessing intraocular pressure by rebound tonometer in rats with an air-filled anterior chamber.

作者信息

Lee Eun Ji, Park Ki Ho, Kim Dong Myung, Yoo Young Cheol, Kang Shin Hee, Kim Yu Jeong

机构信息

Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea.

Seoul Artificial Eye Center, Seoul National University Hospital Clinical Research Institute, Seoul, Korea.

出版信息

Jpn J Ophthalmol. 2008 Nov-Dec;52(6):500-503. doi: 10.1007/s10384-008-0591-1. Epub 2008 Dec 17.

DOI:10.1007/s10384-008-0591-1

PMID:19089574

Abstract

PURPOSE

To compare rebound tonometer and cannulation as methods for measuring intraocular pressure (IOP) in rats.

METHODS

The accuracy of the TonoLab rebound tonometer was determined in eight cannulated rat eyes. IOP was manipulated by changing air pressure from 20 to 100 mmHg at 10-mmHg intervals, and the IOP was measured with the rebound tonometer at each level. The average value of three repeated pressure readings was recorded. Correlation analysis and comparison with the Bland and Altman method were performed. The intraclass correlation coefficient was calculated to assess intraoperator variability.

RESULTS

The IOP values measured with the TonoLab rebound tonometer were well correlated with the actual IOP (r (2) = 0.963, P = 0.01). The mean of the difference between the rebound tonometer and actual (cannulation) IOP was 7.41 +/- 7.87%. The intraclass correlation coefficient was 0.9, indicating low intraoperator variability.

CONCLUSIONS

The rebound tonometer showed high accuracy and reliability for IOP measurement in rat eyes.

摘要

目的

比较回弹式眼压计和插管法作为测量大鼠眼压（IOP）的方法。

方法

在八只插管大鼠眼中测定TonoLab回弹式眼压计的准确性。通过以10 mmHg的间隔将气压从20 mmHg改变至100 mmHg来控制眼压，并在每个水平用回弹式眼压计测量眼压。记录三次重复压力读数的平均值。进行相关性分析并与Bland和Altman方法进行比较。计算组内相关系数以评估操作者内变异性。