Knoshaug Eric P, Zhang Min
National Renewable Energy Laboratory, National Bioenergy Center, Golden, CO 80401, USA.
Appl Biochem Biotechnol. 2009 May;153(1-3):13-20. doi: 10.1007/s12010-008-8460-4. Epub 2008 Dec 17.
Butanol tolerance is a critical factor affecting the ability of microorganisms to generate economically viable quantities of butanol. Current Clostridium strains are unable to tolerate greater than 2% 1-butanol thus membrane or gas stripping technologies to actively remove butanol during fermentation are advantageous. To evaluate the potential of alternative hosts for butanol production, we screened 24 different microorganisms for their tolerance to butanol. We found that in general, a barrier to growth exists between 1% and 2% butanol and few microorganisms can tolerate 2% butanol. Strains of Escherichia coli, Zymomonas mobilis, and non-Saccharomyces yeasts were unable to surmount the 2% butanol growth barrier. Several strains of Saccharomyces cerevisiae exhibit limited growth in 2% butanol, while two strains of Lactobacillus were able to tolerate and grow in up to 3% butanol.
丁醇耐受性是影响微生物产生具有经济可行性数量丁醇能力的关键因素。当前的梭菌菌株无法耐受超过2%的1-丁醇，因此在发酵过程中采用膜或气提技术来主动去除丁醇具有优势。为了评估替代宿主用于丁醇生产的潜力，我们筛选了24种不同的微生物对丁醇的耐受性。我们发现，一般来说，在1%至2%的丁醇浓度之间存在生长障碍，很少有微生物能够耐受2%的丁醇。大肠杆菌、运动发酵单胞菌和非酿酒酵母菌株无法跨越2%丁醇的生长障碍。几株酿酒酵母在2%丁醇中生长受限，而两株乳酸杆菌能够耐受高达3%的丁醇并在其中生长。
