Emre Irfan, Turgut-Balik Dilek, Sahin Ahmet
Department of Primary Education, Faculty of Education, Firat University, Elazig, Turkey.
Pak J Biol Sci. 2007 Sep 1;10(17):2890-4. doi: 10.3923/pjbs.2007.2890.2894.
In this study, total 8 species (Section Cicercula) belong to 14 different geographic distributions collected from Turkey have been studied for the analysis of seed storage protein profiles to examine their relationship by Sodium Dodecyl Sulphate Polyacrilamide Gel Electrophoresis (SDS-PAGE) technique. Hierarchical cluster analysis and Euclidean distance were used for comparison between species and calculating the genetic resemblance respectively. Dendogram was formed using average linkage. Electrophoretic protein profiles of seed cotyledons were showed that all species formed two clusters. The first one consisted of the L. stenophyllus, L. hirsutus, L. chloranthus, L. cicera and L. sativus second one by three species (L. annuus, L. cassius and L. phaselitanus). In addition, very little differences were observed in total protein profiles of the examined sample species (L. cicera, L. hirsutus and L. chloranthus) from nine geographical regions belong to section Cicercula. Protein amount was found to be highest in L. hirsutus and lowest in L. cicera.
在本研究中，对从土耳其收集的属于14种不同地理分布的总共8个物种（鹰嘴豆组）进行了研究，通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）技术分析种子贮藏蛋白谱，以检验它们之间的关系。分别使用层次聚类分析和欧氏距离对物种间进行比较并计算遗传相似性。采用平均连锁法构建聚类图。种子子叶的电泳蛋白谱显示，所有物种形成了两个聚类。第一个聚类由窄叶羽扇豆、硬毛羽扇豆、绿花羽扇豆、鹰嘴豆和栽培羽扇豆组成，第二个聚类由三个物种（一年生羽扇豆、卡西乌斯羽扇豆和菜豆羽扇豆）组成。此外，在属于鹰嘴豆组的9个地理区域的受试样本物种（鹰嘴豆、硬毛羽扇豆和绿花羽扇豆）的总蛋白谱中观察到非常小的差异。发现硬毛羽扇豆中的蛋白含量最高，而鹰嘴豆中的蛋白含量最低。
