Jesse Patrick, Mottke Gritt, Eberle Jürgen, Seifert Georg, Henze Günter, Prokop Aram
Department of Pediatric Oncology/Hematology, Charité, Universitätsmedizin Berlin, Berlin, Germany.
Pediatr Blood Cancer. 2009 Apr;52(4):464-9. doi: 10.1002/pbc.21905.
Helleborus niger is used in the adjuvant treatment of different tumors in anthroposophical medicine. Indications include various types of brain tumors in children, as well as prostate cancer, leukemia and lymphoma. Our aim was to investigate the therapeutic effects of these extracts apart from the traditional use.
: We used an aqueous whole plant extract of H. niger in different cancer and leukemia cell lines and primary cells of patients with childhood ALL and AML and identified the main mechanisms of action.
A strong inhibition of proliferation is caused by specific apoptosis induction, which is executed via the mitochondrial pathway and caspase-3 processing. Apoptosis could be detected in lymphoma (BJAB), leukemia (Reh, Nalm6, Sup-B15) and melanoma (Mel-HO) cells and overcomes a Bcl-2-mediated block of apoptosis. In primary cells of patients with childhood ALL and AML, which were partly poor responding to doxorubicin and daunorubicin, a strong apoptosis induction was determined. In combination with the vinca alkaloid vincristine, strong synergistic effects were detected in BJAB cells.
We demonstrate in vitro efficacy of H. niger extract in cells of hematological malignancies; these studies should encourage in vivo experiments.
黑嚏根草在人智医学中用于不同肿瘤的辅助治疗。适应症包括儿童的各种类型脑肿瘤，以及前列腺癌、白血病和淋巴瘤。我们的目的是研究这些提取物除传统用途外的治疗效果。
我们在不同的癌细胞系、白血病细胞系以及儿童急性淋巴细胞白血病和急性髓细胞白血病患者的原代细胞中使用了黑嚏根草的全株水提取物，并确定了主要作用机制。
通过特定的凋亡诱导导致强烈的增殖抑制，这是通过线粒体途径和半胱天冬酶-3加工来执行的。在淋巴瘤（BJAB）、白血病（Reh、Nalm6、Sup-B15）和黑色素瘤（Mel-HO）细胞中可检测到凋亡，并且克服了Bcl-2介导的凋亡阻滞。在对阿霉素和柔红霉素部分反应不佳的儿童急性淋巴细胞白血病和急性髓细胞白血病患者的原代细胞中，确定了强烈的凋亡诱导。与长春花生物碱长春新碱联合使用时，在BJAB细胞中检测到强烈的协同作用。
我们证明了黑嚏根草提取物在血液系统恶性肿瘤细胞中的体外疗效；这些研究应鼓励进行体内实验。
