Higuchi Wataru, Muramatsu Mineo, Dohmae Soshi, Takano Tomomi, Isobe Hirokazu, Yabe Shizuka, Da Shi, Baranovich Tatiana, Yamamoto Tatsuo
Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
Microbiol Immunol. 2008 Nov;52(11):559-63. doi: 10.1111/j.1348-0421.2008.00065.x.
The use of probiotics such as Lactobacillus in animal feeds has gained popularity in recent years. In this study the 16S rRNA gene sequence of L. acidophilus in two commercial agents which have been used in animal feeds, LAB-MOS and Ghenisson 22, was determined. Phylogenetic tree analysis revealed that the two agents, strain MNFLM01 in LAB-MOS and strain GAL-2 in Ghenisson 22, belonged to L. rhamnosus (a member of the L. casei group) and L. johnsonii (a member of the L. acidophilus group), respectively. Biochemical tests assigned the two as L. rhamnosus and ambiguously as L. acidophilus. The data suggest that 16S rRNA gene sequence analysis provides more accurate identification of Lactobacillus species than biochemical tests and would allow quality assurance of relevant commercial products. The 16S rRNA gene sequences of strains MNFLM01 and GAL-2 determined in this study have been submitted to the DDBJ/EMBL/GenBank accession numbers under accession numbers AB288235 and AB295648, respectively.
近年来，在动物饲料中使用如乳酸杆菌等益生菌已变得流行起来。在本研究中，测定了两种已用于动物饲料的商业制剂LAB-MOS和Ghenisson 22中嗜酸乳杆菌的16S rRNA基因序列。系统发育树分析表明，这两种制剂，LAB-MOS中的菌株MNFLM01和Ghenisson 22中的菌株GAL-2，分别属于鼠李糖乳杆菌（干酪乳杆菌群的一员）和约氏乳杆菌（嗜酸乳杆菌群的一员）。生化试验将二者分别鉴定为鼠李糖乳杆菌和约氏乳杆菌（存在一定模糊性）。数据表明，16S rRNA基因序列分析比生化试验能更准确地鉴定乳酸杆菌物种，并且能确保相关商业产品的质量。本研究中测定的菌株MNFLM01和GAL-2的16S rRNA基因序列已分别以登录号AB288235和AB295648提交至DDBJ/EMBL/GenBank。
