Botina S G, Klimina K M, Koroban N V, Amerkhanova A M, Zinchenko V V, Danilenko V N
Genetika. 2010 Nov;46(11):1485-92.
Thirteen strains of industrial bacterial cultures of the genus Lactobacillus (from a collection of Gabrichevsky Research Institute of Epidemiology and Microbiology) were studied. These strains were used for decades in Russian Federation for food and drug production, as ferments for lactic acid products, for production of probiotics, biologically active and veterinary preparations. Complex analysis of data on cultures obtained using microbiological and molecular-genetic methods was conducted for the first time. Biochemical characteristics of these cultures were studied and the sequence of the proximal region of 16S ribosomal RNA gene was determined. The employment of the test system API-50CHL was shown to broaden the opportunities of a more accurate biochemical identification of bacteria belonging to the genus Lactobacillus, in comparison with the set ANAEROTEST-23. According to the results obtained in a comparative analysis of nucleotide sequences of 16S rRNA gene, all strains examined show 97-99% homology of the proximal region of this gene with that of the type representatives of studied species. These data allowed taxonomic reclassification of the species position of cultures with consideration of the more advanced level of systematics. Nucleotide sequences of gene fragments of examined lactobacilli strains were recorded in NCBI database (accession numbers of deposits GU560031, GU560032, GU560033, GU560034, GU560035, GU560036, GU560037, GU560038, GU560039, GU560040, GU560041, GU560042, GU560043).
对13株乳酸杆菌属工业细菌培养物（来自加布里切夫斯基流行病学与微生物学研究所的菌种保藏中心）进行了研究。这些菌株在俄罗斯联邦已被用于食品和药品生产数十年，用作乳酸产品的发酵剂、益生菌、生物活性制剂和兽用制剂的生产原料。首次使用微生物学和分子遗传学方法对这些培养物的数据进行了综合分析。研究了这些培养物的生化特性，并测定了16S核糖体RNA基因近端区域的序列。结果表明，与ANAEROTEST - 23试剂盒相比，使用API - 50CHL测试系统可拓宽对乳酸杆菌属细菌进行更准确生化鉴定的机会。根据16S rRNA基因核苷酸序列比较分析的结果，所有检测菌株该基因近端区域与所研究物种模式代表的同源性为97 - 99%。这些数据有助于在考虑更先进分类系统水平的情况下对培养物的物种地位进行分类重新划分。所检测乳酸杆菌菌株的基因片段核苷酸序列已记录在NCBI数据库中（保藏登录号为GU560031、GU560032、GU560033、GU560034、GU560035、GU560036、GU560037、GU560038、GU560039、GU560040、GU560041、GU560042、GU560043）。
