Bahler D W, Campbell M J, Hart S, Miller R A, Levy S, Levy R
Department of Medicine, Stanford University School of Medicine, CA.
Blood. 1991 Sep 15;78(6):1561-8.
Thirty-six randomly selected cases of low grade follicular lymphoma (FL) were analyzed for Ig heavy chain variable region (VH) gene expression. Assignment to one of the six human VH gene families (VH1 to VH6) was made with a polymerase chain reaction-based technique using family-specific leader primers. The frequency of VH family use in FL was found to be similar to that reported for normal peripheral blood lymphocytes and is therefore also roughly proportional to VH family size. To evaluate expression within an individual family, all of the lymphoma VH genes from the middle size VH4 family were sequenced and compared with previously published sequences. Of these eight lymphoma VH sequences, six were most closely related to just two of the 10 known functional VH4 germline genes. Nonrandom usage by FL of the JH3, JH4, and JH5 joining segments was also observed. Nucleotide sequences were also determined for 10 randomly selected lymphoma VH genes from the large VH3 family. With one possible exception, none of these lymphoma VH sequences appear to represent any of the VH3 genes that may be preferentially used in the fetal repertoire.
对36例随机选取的低级别滤泡性淋巴瘤（FL）病例进行了免疫球蛋白重链可变区（VH）基因表达分析。采用基于聚合酶链反应的技术，使用家族特异性前导引物将其分配到六个人类VH基因家族（VH1至VH6）之一。发现FL中VH家族的使用频率与正常外周血淋巴细胞的报道相似，因此也大致与VH家族大小成比例。为了评估单个家族内的表达情况，对中等大小的VH4家族中的所有淋巴瘤VH基因进行了测序，并与先前发表的序列进行了比较。在这八个淋巴瘤VH序列中，六个与10个已知功能性VH4种系基因中的仅两个关系最为密切。还观察到FL对JH3、JH4和JH5连接片段的非随机使用。还测定了从大型VH3家族中随机选择的10个淋巴瘤VH基因的核苷酸序列。除了一个可能的例外，这些淋巴瘤VH序列似乎都不代表胎儿库中可能优先使用的任何VH3基因。
