Takacs Peter, Nassiri Mehdi, Gualtieri Marc, Candiotti Keith, Medina Carlos A
Department of Obstetrics and Gynecology (D-50), Jackson Memorial Hospital, University of Miami, Miller School of Medicine, PO Box 016960, Miami, FL 33010, USA.
Reprod Sci. 2009 May;16(5):447-52. doi: 10.1177/1933719108328611. Epub 2008 Dec 17.
The purpose of this study was to compare the smooth muscle content and apoptosis of the uterosacral ligament in women with and without uterine prolapse.
Uterosacral ligaments were sampled in women with (n = 9) or without (n = 9) uterine prolapse undergoing hysterectomy. Smooth muscle of the uterosacral ligament was identified by immunohistochemistry. Digital image analysis was used to determine the fractional area of smooth muscle in the histologic cross sections. Apoptosis was assessed by terminal deoxynucelotidyl-transferase-mediated dUTP nick-end-labeling method.
The fractional area of nonvascular smooth muscle in the uterosacral ligament of women with uterine prolapse was significantly decreased compared to women without prolapse (0.32 +/- 0.12 vs. 0.42 +/- 0.03, P = .02) and the apoptotic index was significantly higher compared to women without prolapse (0.20 +/- 0.06 vs. 0.08 +/- 0.04, P < .01).
The fraction of smooth muscle in the uterosacral ligaments is significantly decreased, and the rate of apoptosis is higher in women with uterine prolapse compared to women without prolapse.
本研究旨在比较有和没有子宫脱垂的女性骶子宫韧带的平滑肌含量及细胞凋亡情况。
在接受子宫切除术的有子宫脱垂（n = 9）和无子宫脱垂（n = 9）的女性中采集骶子宫韧带样本。通过免疫组织化学鉴定骶子宫韧带的平滑肌。使用数字图像分析确定组织学横截面中平滑肌的面积分数。通过末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法评估细胞凋亡。
与无子宫脱垂的女性相比，有子宫脱垂的女性骶子宫韧带中非血管平滑肌的面积分数显著降低（0.32±0.12对0.42±0.03，P = .02），且细胞凋亡指数显著高于无子宫脱垂的女性（0.20±0.06对0.08±0.04，P <.01）。
与无子宫脱垂的女性相比，有子宫脱垂的女性骶子宫韧带中平滑肌的比例显著降低，且细胞凋亡率更高。
