Tashiro K, Kinashi T, Anderson S J, Lee K H, Tohyama K, Ogawa M, Takahashi M, Kawaichi M, Honjo T
Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.
Immunol Lett. 1991 May;28(2):147-54. doi: 10.1016/0165-2478(91)90113-o.
We compared germline transcript levels of immunoglobulin heavy chain and T cell receptor (TcR) genes in a murine hematopoietic stem cell line, LyD9, and its derivative cell lines. LyD9 cells can be induced to differentiate into at least three lineages, namely, B lymphocyte, macrophage, and granulocyte lineages. Although C mu transcripts were found in stem cells to B lymphocytes, other myeloid-committed cells also expressed significant amounts of C mu transcripts. Germline TcR transcripts did not show good correlation with differentiation potential and stages of hematopoietic cells. During this search we identified a novel germline transcript containing the JH-C microliter sequence in LyD9 and some of its derivative cells. Expression of mRNAs for immunoglobulin- and TcR-associated molecules (lambda 5, MB1 and CD3 delta) was widespread except for lambda 5 mRNA. Among three mRNAs encoding putative recombinase proteins, RAG-1 and RAG-2 mRNAs were not expressed in any cell lines tested, while RBP-2 mRNA was expressed ubiquitously.
我们比较了小鼠造血干细胞系LyD9及其衍生细胞系中免疫球蛋白重链和T细胞受体（TcR）基因的种系转录水平。LyD9细胞可被诱导分化为至少三个谱系，即B淋巴细胞、巨噬细胞和粒细胞谱系。虽然在干细胞到B淋巴细胞中发现了Cμ转录本，但其他髓系定向细胞也表达了大量的Cμ转录本。种系TcR转录本与造血细胞的分化潜能和阶段没有良好的相关性。在这项研究中，我们在LyD9及其一些衍生细胞中鉴定出一种含有JH-Cμ序列的新型种系转录本。除λ5 mRNA外，免疫球蛋白和TcR相关分子（λ5、MB1和CD3δ）的mRNA表达广泛。在三种编码假定重组酶蛋白的mRNA中，RAG-1和RAG-2 mRNA在任何测试的细胞系中均未表达，而RBP-2 mRNA普遍表达。
