通过气相色谱全细胞脂肪酸分析鉴别炭疽芽孢杆菌和蜡样芽孢杆菌。
Differentiation of Bacillus anthracis from Bacillus cereus by gas chromatographic whole-cell fatty acid analysis.
作者信息
Lawrence D, Heitefuss S, Seifert H S
机构信息
Institute for Tropical Animal Health, Georg-August-University, Gottingen, Federal Republic of Germany.
出版信息
J Clin Microbiol. 1991 Jul;29(7):1508-12. doi: 10.1128/jcm.29.7.1508-1512.1991.
Abstract
Three strains of Bacillus anthracis and seven strains of Bacillus cereus were grown on complex medium and on synthetic medium. Gas chromatographic analysis of whole-cell fatty acids of strains grown on complex medium gave nearly identical fatty acid patterns. Fatty acid patterns of strains grown on synthetic medium showed a high content of branched-chain fatty acids. Significant differences between the fatty acid patterns of the two species were found. Odd iso/anteiso fatty acid ratios were about equal in B. anthracis strains, whereas in B. cereus strains the fractions of iso acids were at least twice as high as the fractions of anteiso acids. The method described herein is used in our diagnostic laboratory to help differentiate between these two species.
摘要
三株炭疽芽孢杆菌和七株蜡样芽孢杆菌分别在复合培养基和合成培养基上培养。对在复合培养基上生长的菌株的全细胞脂肪酸进行气相色谱分析，得到的脂肪酸图谱几乎相同。在合成培养基上生长的菌株的脂肪酸图谱显示支链脂肪酸含量很高。发现这两个菌种的脂肪酸图谱存在显著差异。炭疽芽孢杆菌菌株的奇数异/反异脂肪酸比率大致相等，而在蜡样芽孢杆菌菌株中，异酸的比例至少是反异酸比例的两倍。本文所述方法在我们的诊断实验室中用于帮助区分这两个菌种。
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5
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