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Differentiation of Bacillus anthracis from Bacillus cereus by gas chromatographic whole-cell fatty acid analysis.通过气相色谱全细胞脂肪酸分析鉴别炭疽芽孢杆菌和蜡样芽孢杆菌。
J Clin Microbiol. 1991 Jul;29(7):1508-12. doi: 10.1128/jcm.29.7.1508-1512.1991.
2
Fatty acids in the genus Bacillus. II. Similarity in the fatty acid compositions of Bacillus thuringiensis, Bacillus anthracis, and Bacillus cereus.芽孢杆菌属中的脂肪酸。II. 苏云金芽孢杆菌、炭疽芽孢杆菌和蜡样芽孢杆菌脂肪酸组成的相似性。
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Fatty acids in the genus Bacillus. I. Iso- and anteiso-fatty acids as characteristic constituents of lipids in 10 species.芽孢杆菌属中的脂肪酸。I. 异脂肪酸和反异脂肪酸作为10个物种中脂质的特征成分。
J Bacteriol. 1967 Mar;93(3):894-903. doi: 10.1128/jb.93.3.894-903.1967.
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Use of fatty acid profiles to identify food-borne bacterial pathogens and aerobic endospore-forming bacilli.利用脂肪酸谱鉴定食源性病原体和需氧芽孢杆菌。
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Determination of fatty acid compositions of Bacillus cereus and related bacteria: a rapid gas chromatographic method using a glass capillary column.蜡样芽孢杆菌及相关细菌脂肪酸组成的测定：一种使用玻璃毛细管柱的快速气相色谱法。
Appl Environ Microbiol. 1978 Feb;35(2):453-5. doi: 10.1128/aem.35.2.453-455.1978.
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Differentiation of dairy strains of the Bacillus cereus group by phage typing, minimum growth temperature, and fatty acid analysis.通过噬菌体分型、最低生长温度和脂肪酸分析对蜡样芽孢杆菌群的乳制品菌株进行鉴别
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Use of fatty acid methyl ester profiles for discrimination of Bacillus cereus T-strain spores grown on different media.利用脂肪酸甲酯图谱区分不同培养基上生长的蜡样芽胞杆菌 T 株孢子。
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Determination of carbohydrate profiles of Bacillus anthracis and Bacillus cereus including identification of O-methyl methylpentoses by using gas chromatography-mass spectrometry.利用气相色谱-质谱联用技术测定炭疽芽孢杆菌和蜡样芽孢杆菌的碳水化合物谱，包括O-甲基甲基戊糖的鉴定。
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Arbitrary primer polymerase chain reaction, a powerful method to identify Bacillus thuringiensis serovars and strains.任意引物聚合酶链反应，一种鉴定苏云金芽孢杆菌血清型和菌株的强大方法。
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Bacillus cereus and related species.蜡样芽孢杆菌及相关菌种。
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本文引用的文献

1
[Bacterial differential diagnosis of the anthrax by means of a new specific test called the string-of perls-test].[通过一种名为珍珠串试验的新型特异性检测方法对炭疽进行细菌鉴别诊断]
Zentralbl Bakteriol Orig. 1953 Sep 5;159(8):494-500.
2
Identification of Bacillus strains using the API system.使用API系统鉴定芽孢杆菌菌株。
J Gen Microbiol. 1984 Jul;130(7):1871-82. doi: 10.1099/00221287-130-7-1871.
3
Factors affecting the relative ratio of fatty acids in Bacillus cereus.影响蜡样芽孢杆菌中脂肪酸相对比例的因素。
Can J Microbiol. 1971 Feb;17(2):269-75. doi: 10.1139/m71-045.
4
Fatty acids in the genus Bacillus. II. Similarity in the fatty acid compositions of Bacillus thuringiensis, Bacillus anthracis, and Bacillus cereus.芽孢杆菌属中的脂肪酸。II. 苏云金芽孢杆菌、炭疽芽孢杆菌和蜡样芽孢杆菌脂肪酸组成的相似性。
J Bacteriol. 1968 Jun;95(6):2210-6. doi: 10.1128/jb.95.6.2210-2216.1968.
5
Fatty acids in the genus Bacillus. I. Iso- and anteiso-fatty acids as characteristic constituents of lipids in 10 species.芽孢杆菌属中的脂肪酸。I. 异脂肪酸和反异脂肪酸作为10个物种中脂质的特征成分。
J Bacteriol. 1967 Mar;93(3):894-903. doi: 10.1128/jb.93.3.894-903.1967.
6
Selective medium for Bacillus anthracis.炭疽芽孢杆菌的选择性培养基。
J Bacteriol. 1966 Sep;92(3):784-6. doi: 10.1128/jb.92.3.784-786.1966.
7
Differential media for the identification of Bacillus anthracis.用于鉴定炭疽芽孢杆菌的鉴别培养基。
J Bacteriol. 1965 Dec;90(6):1778-83. doi: 10.1128/jb.90.6.1778-1783.1965.
8
DNA competition studies within the Bacillus cereus group of bacilli.蜡样芽孢杆菌群内的DNA竞争研究。
J Gen Microbiol. 1972 Nov;73(2):257-65. doi: 10.1099/00221287-73-2-257.
9
Production and purification of anthrax toxin.炭疽毒素的生产与纯化。
Methods Enzymol. 1988;165:103-16. doi: 10.1016/s0076-6879(88)65019-1.
10
[A new method to classify pathogene anaerobes, the statistical evaluation of quantitative determined patterns from metabolic fatty acids (author's transl)].[一种对致病性厌氧菌进行分类的新方法，即对代谢脂肪酸定量测定模式的统计学评估（作者译）]
Zentralbl Bakteriol Orig A. 1979 Mar;243(1):82-101.

通过气相色谱全细胞脂肪酸分析鉴别炭疽芽孢杆菌和蜡样芽孢杆菌。

Differentiation of Bacillus anthracis from Bacillus cereus by gas chromatographic whole-cell fatty acid analysis.

作者信息

Lawrence D, Heitefuss S, Seifert H S

机构信息

Institute for Tropical Animal Health, Georg-August-University, Gottingen, Federal Republic of Germany.

出版信息

J Clin Microbiol. 1991 Jul;29(7):1508-12. doi: 10.1128/jcm.29.7.1508-1512.1991.

DOI:10.1128/jcm.29.7.1508-1512.1991
PMID:1909348
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC270143/
Abstract

Three strains of Bacillus anthracis and seven strains of Bacillus cereus were grown on complex medium and on synthetic medium. Gas chromatographic analysis of whole-cell fatty acids of strains grown on complex medium gave nearly identical fatty acid patterns. Fatty acid patterns of strains grown on synthetic medium showed a high content of branched-chain fatty acids. Significant differences between the fatty acid patterns of the two species were found. Odd iso/anteiso fatty acid ratios were about equal in B. anthracis strains, whereas in B. cereus strains the fractions of iso acids were at least twice as high as the fractions of anteiso acids. The method described herein is used in our diagnostic laboratory to help differentiate between these two species.

摘要

三株炭疽芽孢杆菌和七株蜡样芽孢杆菌分别在复合培养基和合成培养基上培养。对在复合培养基上生长的菌株的全细胞脂肪酸进行气相色谱分析，得到的脂肪酸图谱几乎相同。在合成培养基上生长的菌株的脂肪酸图谱显示支链脂肪酸含量很高。发现这两个菌种的脂肪酸图谱存在显著差异。炭疽芽孢杆菌菌株的奇数异/反异脂肪酸比率大致相等，而在蜡样芽孢杆菌菌株中，异酸的比例至少是反异酸比例的两倍。本文所述方法在我们的诊断实验室中用于帮助区分这两个菌种。