植物生长调节剂对麻疯树愈伤组织培养中脂肪酸组成的影响。

Effect of plant growth regulators on fatty acids composition in Jatropha curcas L. callus culture.

作者信息

Hernandez Ludwi Rodríguez, Mendiola Martha A Rodríguez, Castro Carlos Arias, Gutiérrez-Miceli Federico A

机构信息

Area de Posgrado en Ciencias en Agrobiotecnología. Instituto Tecnológico de Tlajomulco.

出版信息

J Oleo Sci. 2015;64(3):325-30. doi: 10.5650/jos.ess14206. Epub 2015 Feb 9.

DOI:10.5650/jos.ess14206

PMID:25757437

Abstract

The influence of Naphtaleneacetic acid (NAA) and 6-Benzylaminopurine (BAP) on callus formation, its morphology and fatty acids profile were examined from Jatropha curcas L. Embryo from seeds of J. curcas L. were sown in Murashige and skoog (MS) medium with NAA and BAP. All treatments induced callus formation, however callus morphology was different in most of the treatments. Higher callus biomass was presented with 1.0 NAA + 0.5 BAP mg/L. Plant growth regulators modifies the fatty acids profile in callus of J. curcas L. BAP was induced linoleic and linolenic acids.

摘要

研究了萘乙酸（NAA）和6-苄基氨基嘌呤（BAP）对麻疯树愈伤组织形成、其形态及脂肪酸谱的影响。将麻疯树种子的胚播种于添加了NAA和BAP的Murashige和skoog（MS）培养基中。所有处理均诱导了愈伤组织的形成，但大多数处理中愈伤组织的形态不同。在1.0 NAA + 0.5 BAP mg/L处理下出现了更高的愈伤组织生物量。植物生长调节剂改变了麻疯树愈伤组织中的脂肪酸谱。BAP诱导了亚油酸和亚麻酸的产生。