Rancić D, Quarrie S Pekić, Terzić M, Savić S, Stikić R
Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Zemun, Serbia.
J Microsc. 2008 Dec;232(3):618-22. doi: 10.1111/j.1365-2818.2008.02127.x.
The xylem hydraulic connection between shoot and fruits has previously been investigated, but contradictory conclusions were drawn about the presence of a flow resistance barrier in the pedicel. In this paper we were studying effect of the drought on the functional xylem vessels in the pedicels of tomato fruit. Commercial tomato genotype was grown in cabinet conditions under two watering regimes (full and deficit irrigation). An aqueous solution of eosin Y were used to visualize the path of water movement through tomato fruit pedicel and fluorescence microscopy observations were done on transversal and longitudinal sections. Dye uptake studies suggested that in well watered plants and in plants exposed to drought, a large majority of xylem vessels are not functional in water transport. Reduced-irrigation treatment significantly altered number and width of functional xylem elements in the fruit pedicel, especially in the abscission zone. This indicates that drought modifies xylem architecture and, thus, environmentally produced change in the hydraulic property of pedicel may affect fruit development.
此前已对茎与果实之间的木质部水力连接进行了研究，但关于花梗中是否存在流动阻力屏障得出了相互矛盾的结论。在本文中，我们研究了干旱对番茄果实花梗中功能性木质部导管的影响。将商业番茄基因型种植在温室条件下的两种浇水制度（充分灌溉和亏缺灌溉）下。使用伊红Y水溶液来观察水分通过番茄果实花梗的移动路径，并对横切面和纵切面进行荧光显微镜观察。染料吸收研究表明，在浇水良好的植株和遭受干旱的植株中，绝大多数木质部导管在水分运输中不起作用。减少灌溉处理显著改变了果实花梗中功能性木质部元素的数量和宽度，尤其是在离层区。这表明干旱会改变木质部结构，因此，环境引起的花梗水力特性变化可能会影响果实发育。
