Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080, Belgrade, Serbia.
Protoplasma. 2010 Oct;246(1-4):25-31. doi: 10.1007/s00709-010-0115-y. Epub 2010 Feb 18.
The abscission zone in fruit pedicels plays an important role in affecting not only water uptake in the developing fruit, but also in the transport of chemical signals from root to shoot. In order to characterize the hydraulic network of tomato fruit pedicels, we applied various techniques, including light, fluorescence microscopy, electron microscopy, maceration, tissue clearing, and X-ray computed tomography. Because of significant changes in xylem anatomy, the abscission zone in tomato fruit pedicels is illustrated to show a clear reduction in hydraulic conductance. Based on anatomical measurements, the theoretical axial xylem conductance was calculated via the Hagen-Poiseuille law, suggesting that the hydraulic resistance of the abscission zone increases at least two orders of magnitude compared to the pedicel zone near the stem. The advantages and shortcomings of the microscope techniques applied are discussed.
果实柄的离区在影响水分吸收方面起着重要作用，不仅在发育中的果实中，而且在根到茎的化学信号传递中也是如此。为了描述番茄果实柄的水力网络，我们应用了多种技术，包括光、荧光显微镜、电子显微镜、浸没法、组织透明法和 X 射线计算机断层扫描。由于木质部解剖结构的显著变化，番茄果实柄的离区显示出明显的水力导降低。基于解剖学测量，通过哈根-泊肃叶定律计算了理论轴向木质部导，表明离区的水力阻力至少比靠近茎的柄区增加了两个数量级。讨论了所应用显微镜技术的优缺点。
