Dlugosz J W, Korsten M A, Lieber C S
Alcohol Research and Treatment Center, Bronx VA Medical Center, NY 10468.
Life Sci. 1991;49(13):969-78. doi: 10.1016/0024-3205(91)90080-u.
Rats fed ethanol (36% of total calories in a nutritionally adequate liquid diet) for 5 weeks develop functional alterations of hepatic mitochondria and steatosis of the liver. At the fatty liver stage, ADP-stimulated respiration of mitochondria was depressed in ethanol fed rats by 30% (p less than 0.001) with glutamate + malate and by 23% (p less than 0.001) with succinate as substrates. A similar decrease was noted in the respiratory control ratio (RCR) (34% and 29%, respectively). The total lipid content of the liver increased 2.6 fold (p less than 0.001). Mitochondrial dysfunction could be prevented, in part, by the treatment with a synthetic derivative of prostaglandin E1, misoprostol, at a mean daily dose of 80 micrograms/kg of body weight. The RCR with glutamate + malate as substrates was improved by 36% (p less than 0.05). We conclude that misoprostol attenuates several functional alterations in liver mitochondria during alcohol feeding.
给大鼠喂食乙醇（占营养充足的液体饮食中总热量的36%）5周后，会出现肝线粒体功能改变和肝脏脂肪变性。在脂肪肝阶段，以谷氨酸 + 苹果酸为底物时，乙醇喂养的大鼠线粒体中ADP刺激的呼吸作用降低了30%（p < 0.001），以琥珀酸为底物时降低了23%（p < 0.001）。呼吸控制率（RCR）也有类似程度的下降（分别为34%和29%）。肝脏的总脂质含量增加了2.6倍（p < 0.001）。每天以平均80微克/千克体重的剂量用前列腺素E1的合成衍生物米索前列醇进行治疗，可部分预防线粒体功能障碍。以谷氨酸 + 苹果酸为底物时的RCR提高了36%（p < 0.05）。我们得出结论，米索前列醇可减轻酒精喂养期间肝脏线粒体的几种功能改变。
