Yigit N, Aktas A E, Ayyildiz A
Medical Laboratory Department, Atatürk University Health Services Vocational Training School, Erzurum, Turkey.
J Int Med Res. 2008 Nov-Dec;36(6):1378-82. doi: 10.1177/147323000803600627.
The coagulase activities of various Candida spp were assessed using a classical tube test. A total of 125 clinical Candida isolates representing eight species (C. albicans, C. parapsilosis, C. tropicalis, C. glabrata, C. kefyr, Geotrichum candidum, C. krusei and C. guilliermondii) were incubated with rabbit, sheep or human plasma. After 24 h incubation, 29/64 (45.3%) C. albicans isolates and 23/61 (37.7%) non-albicans Candida strains were positive for coagulase in rabbit plasma, whereas 20/64 (31.2%) C. albicans and 6/61 (9.8%) non-albicans Candida isolates were positive for coagulase in sheep plasma. None of the tested Candida isolates reacted positively in human plasma. These results suggest that rabbit plasma is the most appropriate medium for detection of coagulase activity in Candida spp. Variations in coagulase production by Candida spp may be related to their pathogenicity. It is concluded that the laboratory detection of coagulase activity in clinical isolates of Candida spp may help the diagnosis of Candida-related infections.
使用经典试管试验评估了各种念珠菌属的凝固酶活性。总共125株临床念珠菌分离株，代表8个菌种（白色念珠菌、近平滑念珠菌、热带念珠菌、光滑念珠菌、克柔念珠菌、白地霉、克鲁斯念珠菌和季也蒙念珠菌），与兔、羊或人血浆一起孵育。孵育24小时后，64株白色念珠菌分离株中有29株（45.3%）和61株非白色念珠菌菌株中有23株（37.7%）在兔血浆中凝固酶呈阳性，而64株白色念珠菌中有20株（31.2%）和61株非白色念珠菌分离株中有6株（9.8%）在羊血浆中凝固酶呈阳性。所测试的念珠菌分离株在人血浆中均无阳性反应。这些结果表明，兔血浆是检测念珠菌属凝固酶活性的最合适培养基。念珠菌属凝固酶产生的差异可能与其致病性有关。结论是，实验室检测临床念珠菌分离株的凝固酶活性可能有助于念珠菌相关感染的诊断。
