腹股沟斜疝患者腹横筋膜中的胶原蛋白：一项病例对照研究。
Collagen in the transversalis fascia of patients with indirect inguinal hernia: a case-control study.
作者信息
Casanova Antonio Britto, Trindade Eduardo Neubarth, Trindade Manoel Roberto Maciel
机构信息
Department of Surgery, Universidade de Caxias do Sul, Caxias do Sul, Brazil.
出版信息
Am J Surg. 2009 Jul;198(1):1-5. doi: 10.1016/j.amjsurg.2008.07.021. Epub 2008 Dec 18.
BACKGROUND
The aim of this study was to analyze the constituents of total and types I and III collagen fibers in the transversalis fascias of patients with indirect inguinal hernias, compared with samples removed from nonherniated cadavers.
METHODS
Biopsy samples from 26 patients and 26 cadavers were analyzed. Hematoxylin-eosin and picrosirius staining techniques were used. The images obtained were analyzed using a video morphometric technique to determine the constituents of total collagen and types I and III collagen in the transversalis fascia. The picrosirius-stained tissues were submitted to observation under polarized-light microscopy.
RESULTS
The results showed 17.3% less total collagen in patients with hernias compared with the control group (P < .01). Type I collagen in patients with indirect inguinal hernias was 23.7% less than the control group (P < .01), type III collagen was 6.4% less in the controls (P < .01).
CONCLUSION
The lower percentages of total collagen and type I collagen in the transversalis fascias of patients with indirect inguinal hernias could be a factor in hernia formation.
背景
本研究旨在分析间接性腹股沟疝患者腹横筋膜中总胶原纤维以及I型和III型胶原纤维的成分，并与非疝性尸体的样本进行比较。
方法
对26例患者和26具尸体的活检样本进行分析。采用苏木精-伊红染色和天狼星红染色技术。使用视频形态计量技术对获得的图像进行分析，以确定腹横筋膜中总胶原以及I型和III型胶原的成分。将天狼星红染色的组织置于偏光显微镜下观察。
结果
结果显示，与对照组相比，疝患者的总胶原减少了17.3%（P <.01）。间接性腹股沟疝患者的I型胶原比对照组少23.7%（P <.01），III型胶原在对照组中少6.4%（P <.01）。
结论
间接性腹股沟疝患者腹横筋膜中总胶原和I型胶原的比例较低可能是疝形成的一个因素。
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